Lipid damage is the best marker of oxidative injury during the cardiac remodeling process induced by tobacco smoke

Author(s):Show less -

Martins Lourenco, Maria Angelica ^[1] ; Braz, Mariana Gobbo ^[2] ; Aun, Aline Garcia ^[2] ; Buzati Pereira, Bruna Leticia ^[1] ; Fernandes, Fabio Henrique ^[3] ; Kazmarek, Elisa Moya ^[1] ; Bachiega, Tatiana Fernanda ^[1] ; Zanati, Silmeia Garcia ^[1] ; Azevedo, Paula Schmidt ^[1] ; Polegato, Bertha Furlan ^[1] ; Henrique Fernandes, Ana Angelica ^[4] ; Rupp de Paiva, Sergio Alberto ^[1] ; Mamede Zornoff, Leonardo Antonio ^[1] ; Minicucci, Marcos Ferreira ^{[1, 5]}

Affiliation

^[1] Sao Paulo State Univ, UNESP, Botucatu Med Sch, Internal Med Dept, Botucatu, SP - Brazil

^[2] Sao Paulo State Univ, UNESP, Botucatu Med Sch, Dept Anesthesiol, Botucatu, SP - Brazil

^[3] Sao Paulo State Univ, UNESP, Inst Biol Sci, Dept Genet, Botucatu, SP - Brazil

^[4] Sao Paulo State Univ, UNESP, Botucatu Med Sch, Chem & Biochem Dept, Inst Biol Sci, Botucatu, SP - Brazil

^[5] Fac Med Botucatu, Dept Clin Med, Rubiao Jr S-N, BR-18618000 Botucatu, SP - Brazil

• Document type: Journal article
• Source: BMC PHARMACOLOGY & TOXICOLOGY; v. 19, NOV 16 2018.
• Web of Science Citations: 0

Abstract

BackgroundOxidative stress is one potential mechanism that explain the direct effects of smoking on cardiac remodeling process. However, no study has compared different myocardial products of macromolecule oxidation after tobacco smoke exposure. Thus, the aim of this study was to investigate the lipid hydroperoxide (LH) levels, protein carbonyl concentrations and DNA damage in cardiac tissue of rats exposed to tobacco smoke.MethodsMale Wistar rats were divided into two groups: group C (control, n=14) composed of animals not exposed to cigarette smoke; group ETS (exposed to tobacco smoke, n=14) composed by animals exposed to cigarette smoke. The animals were exposed to 2month of ETS and morphological, biochemical and functional analyses were performed.ResultsCardiac cotinine levels were elevated in the ETS group. In addition, the myocyte cross-sectional area was higher in the ETS group. (C=266.623.2m(2) and ETS=347.5 +/- 15.1m(2), p<0.001). Cardiac LH was higher in the ETS group than in group C (C=196.4 +/- 51.5nmol/g and ETS=331.9 +/- 52.9nmol/g, p<0.001). However, there were no between-group differences in cardiac protein carbonyl concentration or DNA damage.Conclusions Therefore, our results suggest that, in this model, lipid damage is a good marker of oxidative damage during the cardiac remodeling process induced by 2months of exposure to tobacco smoke. (AU)

• FAPESP's process: 14/21310-0 - Influence of caja supplementation on cardiac remodeling induced by cigarette smoke exposure in rats
• Grantee: Maria Angelica Martins Lourenço
• Support Opportunities: Scholarships in Brazil - Master