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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Preparation of Magnetic Cross-Linked Amyloglucosidase Aggregates: Solving Some Activity Problems

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Author(s):
Amaral-Fonseca, Murilo [1] ; Kopp, Willian [2] ; Camargo Giordano, Raquel de Lima [1] ; Fernandez-Lafuente, Roberto [3] ; Tardioli, Paulo Waldir [1]
Total Authors: 5
Affiliation:
[1] Fed Univ Sao Carlos PPGEQ UFSCar, Dept Chem Engn, Postgrad Program Chem Engn, Rod Washington Luiz, Km 235, BR-13565905 Sao Carlos, SP - Brazil
[2] Kopp Technol KTech, Rua Alfredo Lopes 1717, BR-13560460 Sao Carlos, SP - Brazil
[3] ICP CSIC, Dept Biocatalis, Campus UAM CSIC, Madrid 28040 - Spain
Total Affiliations: 3
Document type: Journal article
Source: CATALYSTS; v. 8, n. 11 NOV 2018.
Web of Science Citations: 7
Abstract

The preparation of Cross-Linked Enzyme Aggregates (CLEAs) is a simple and cost-effective technique capable of generating insoluble biocatalysts with high volumetric activity and improved stability. The standard CLEA preparation consists of the aggregation of the enzyme and its further crosslinking, usually with glutaraldehyde. However, some enzymes have too low a content of surface lysine groups to permit effective crosslinking with glutaraldehyde, requiring co-aggregation with feeders rich in amino groups to aid the formation of CLEAs. The co-aggregation with magnetic particles makes their handling easier. In this work, CLEAs of a commercial amyloglucosidase (AMG) produced by Aspergillus niger were prepared by co-aggregation in the presence of polyethyleneimine (PEI) or starch with aminated magnetic nanoparticles (MNPs) or bovine serum albumin (BSA). First, CLEAs were prepared only with MNPs at different glutaraldehyde concentrations, yielding a recovered activity of around 20%. The addition of starch during the precipitation and crosslinking steps nearly doubled the recovered activity. Similar recovered activity (around 40%) was achieved when changing starch by PEI. Moreover, under the same conditions, AMG co-aggregated with BSA was also synthesized, yielding CLEAs with very similar recovered activity. Both CLEAs (co-aggregated with MNPs or BSA) were four times more stable than the soluble enzyme. These CLEAs were evaluated in the hydrolysis of starch at typical industrial conditions, achieving more than 95% starch-to-glucose conversion, measured as Dextrose Equivalent (DE). Moreover, both CLEAS could be reused for five cycles, maintaining a DE of around 90%. Although both CLEAs had good properties, magnetic CLEAs could be more attractive for industrial purposes because of their easy separation by an external magnetic field, avoiding the formation of clusters during the filtration or centrifugation recovery methods usually used. (AU)

FAPESP's process: 16/10636-8 - From the cell factory to the Biodiesel-Bioethanol integrated biorefinery: a systems approach applied to complex problems in micro and macroscales
Grantee:Roberto de Campos Giordano
Support Opportunities: Program for Research on Bioenergy (BIOEN) - Thematic Grants