Advanced search
Start date
(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Zebrafish larvae heartbeat detection from body deformation in low resolution and low frequency video

Full text
Xing, Qi [1] ; Huynh, Victor [2] ; Parolari, Thales Guimaraes [3] ; Maurer-Morelli, Claudia Vianna [3] ; Peixoto, Nathalia [4] ; Wei, Qi [2]
Total Authors: 6
[1] George Mason Univ, Dept Comp Sci, Fairfax, VA 22030 - USA
[2] George Mason Univ, Bioengn Dept, Fairfax, VA 22030 - USA
[3] Univ Estadual Campinas, Sch Med Sci, Dept Med Genet, Campinas, SP - Brazil
[4] Elect & Comp Engn Dept, Fairfax, VA - USA
Total Affiliations: 4
Document type: Journal article
Source: MEDICAL & BIOLOGICAL ENGINEERING & COMPUTING; v. 56, n. 12, p. 2353-2365, DEC 2018.
Web of Science Citations: 2

Zebrafish (Danio rerio) is a powerful animal model used in many areas of genetics and disease research. Despite its advantages for cardiac research, the heartbeat pattern of zebrafish larvae under different stress conditions is not well documented quantitatively. Several effective automated heartbeat detection methods have been developed to reduce the workload for larva heartbeat analysis. However, most require complex experimental setups and necessitate direct observation of the larva heart. In this paper, we propose the Zebrafish Heart Rate Automatic Method (Z-HRAM), which detects and tracks the heartbeats of immobilized, ventrally positioned zebrafish larvae without direct larva heart observation. Z-HRAM tracks localized larva body deformation that is highly correlated with heart movement. Multiresolution dense optical flow-based motion tracking and principal component analysis are used to identify heartbeats. Here, we present results of Z-HRAM on estimating heart rate from video recordings of seizure-induced larvae, which were of low resolution (1024x760) and low frame rate (3 to 4fps). Heartbeats detected from Z-HRAM were shown to correlate reliably with those determined through corresponding electrocardiogram and manual video inspection. We conclude that Z-HRAM is a robust, computationally efficient, and easily applicable tool for studying larva cardiac function in general laboratory conditions. Flowchart of the automatic zebrafish heartbeat detection (AU)

FAPESP's process: 13/07559-3 - BRAINN - The Brazilian Institute of Neuroscience and Neurotechnology
Grantee:Fernando Cendes
Support type: Research Grants - Research, Innovation and Dissemination Centers - RIDC
FAPESP's process: 14/15640-8 - Functional studies of genes of inflammation and microRNAs related to seizures using the zebrafish model
Grantee:Cláudia Vianna Maurer Morelli
Support type: Regular Research Grants