Genetic variants in ACTN3 and MYO1H are associated with sagittal and vertical craniofacial skeletal patterns

Author(s):Show less -

Cunha, Arthur ^[1] ; Nelson-Filho, Paulo ^[1] ; Maranon-Vasquez, Guido Artemio ^[1] ; de Carvalho Ramos, Alice Gomes ^{[1, 2]} ; Dantas, Beatriz ^{[1, 2]} ; Sebastiani, Aline Monise ^[3] ; Silverio, Felipe ^[3] ; Omori, Marjorie Ayumi ^[1] ; Rodrigues, Amanda Silva ^[4] ; Teixeira, Ellen Cardoso ^{[5, 6]} ; Levy, Simone Carvalho ^{[5, 6]} ; de Araujo, Marcelo Calvo ^[7] ; Nakane Matsumoto, Mirian Aiko ^[1] ; Romano, Fabio Lourenco ^[1] ; Antunes, Livia Azeredo A. ^{[5, 6]} ; da Costa, Delson Joao ^[4] ; Scariot, Rafaela ^{[3, 4]} ; Antunes, Leonardo Santos ^{[5, 6]} ; Vieira, Alexandre R. ^[8] ; Kuchler, Erika C. ^{[3, 1]}

Affiliation

^[1] Univ Sao Paulo, Sch Dent Ribeirao Preto, Dept Pediat Dent, Ave Cafe S-N Campus USP, BR-14040904 Ribeirao Preto, SP - Brazil

^[2] Amazonian Educ Inst, Rua Maceio 861, BR-69057010 Manaus, AM - Brazil

^[3] Univ Curitiba, Rua Prof Pedro Viriato Parigot de Souza 5300, BR-81200452 Curitiba, PR - Brazil

^[4] Univ Fed Parana, Dept Oral & Maxillofacial Surg, Ave Prefeito Lothario Meisser 632, BR-80210170 Curitiba, PR - Brazil

^[5] Fluminense Fed Univ, Sch Dent, Rua Sao Paulo 28, Campus Valonguinho, BR-24020150 Niteroi, RJ - Brazil

^[6] Fluminense Fed Univ, Sch Dent, Rua Doutor Silvio Henrique Braune 22, BR-28625650 Nova Friburgo, RJ - Brazil

^[7] Smile Grad Sch & Clin, Rua Jose Clemente 94, BR-24020115 Niteroi, RJ - Brazil

^[8] Univ Pittsburgh, Sch Dent Med, Dept Oral Biol, 412 Salk Pavil, 335 Sutherland St, Pittsburgh, PA 15261 - USA

• Document type: Journal article
• Source: ARCHIVES OF ORAL BIOLOGY; v. 97, p. 85-90, JAN 2019.
• Web of Science Citations: 2

Abstract

Objective: This study aimed to evaluate the association of genetic variants inACTN3 and MYO1H with craniofacial skeletal patterns in Brazilians. Design: This cross-sectional study enrolled orthodontic and orthognathic patients selected from 4 regions of Brazil. Lateral cephalograms were used and digital cephalometric tracings and analyzes were performed for craniofacial phenotype determination. Participants were classified according to the skeletal malocclusion in Class I, II or III; and according to the facial type in Mesofacial, Dolichofacial or Brachyfacial. Genomic DNA was extracted from saliva samples containing exfoliated buccal epithelial cells and analyzed for genetic variants inACTN3 (rs678397 and rs1815739) and MYO1H (rs10850110) by real-time PCR. Chi-square or Fisher's exact tests were used for statistical analysis (alpha = 5%). Results: A total of 646 patients were included in the present study. There was statistically significant association of the genotypes and/or alleles distributions with the skeletal malocclusion (sagittal skeletal pattern) and facial type (vertical pattern) for the variants assessed inACTN3 (P < 0.05). For the genetic variant evaluated in MYO1H, there was statistically significant difference between the genotypes frequencies for skeletal Class I and Class II (P < 0.05). The reported associations were different depending on the region evaluated. Conclusion: ACTN3 and MYO1H are associated with sagittal and vertical craniofacial skeletal patterns in Brazilian populations. (AU)

• FAPESP's process: 15/06866-5 - Evaluation of the role of estrogen in dentofacial development
• Grantee: Erika Calvano Kuchler
• Support Opportunities: Research Grants - Young Investigators Grants