In vitro evaluation of essential oils for potential antibacterial effects against Xylella fastidiosa

Author(s)

Santiago, Mariana Brentini ^[1] ; Moraes, Thais da Silva ^[1] ; Massuco, Jaqueline Estetele ^[1] ; Silva, Larissa Oliveira ^[1] ; Lucarini, Rodrigo ^[1] ; da Silva, Danielle Fernandes ^[1] ; Vieira, Tatiana Manzini ^[2] ; Miller Crotti, Antonio Eduardo ^[2] ; Gomes Martins, Carlos Henrique ^[1]

Affiliation

^[1] Univ Franca, Appl Microbiol Res Lab, Nucleus Res Sci & Technol, Franca, SP - Brazil

^[2] Univ Sao Paulo, Dept Quim, Fac Filosofia Ciencias & Letras Ribeirao Preto, Ribeirao Preto - Brazil

• Document type: Journal article
• Source: JOURNAL OF PHYTOPATHOLOGY; v. 166, n. 11-12, p. 790-798, DEC 2018.
• Web of Science Citations: 1

Abstract

The main pathogen underlying citrus variegated chlorosis (CVC), a citrus fruit disease, is Xylella fastidiosa, a Gram-negative s-shaped bacterium that blocks water and nutrient circulation. Discovering new pesticides to combat CVC have become a challenge. Research into essential oils (EOs) is crucial because these substances can be potentially useful in pest control. This study aims to determine minimum inhibitory concentrations (MIC), to investigate biofilm inhibition capacities through MBIC50 analysis, and to evaluate synergistic effects with streptomycin of 17 EOs against X. fastidiosa. This study also analyses the chemical profiles of the EOs that provided the best antibacterial activity results. The MIC values of the EOs ranged from 125 to 1,000 mu g/ml. Analysis of the MICB50 values of the sandalwood and patchouli EOs revealed biofilm inhibitory activity. No synergistic effects were evident for streptomycin combined with the sandalwood or patchouli EOs: sigma FIC was 1.25 and 1.5, respectively, which denoted indifference. We detected alpha-gurjunene (27.6%), patchouli alcohol (21.6%), alpha-bulsene (17.3%) and alpha-patchoulene (6.3%) as the major patchouli EO constituents. On the basis of our results, the sandalwood and patchouli EOs present antibacterial action and are promising natural sources for the development of new pesticides against X. fastidiosa. (AU)

• FAPESP's process: 12/25237-0 - Kaurane-type diterpenes: activity against anaerobic bacteria and proteomics
• Grantee: Carlos Henrique Gomes Martins
• Support Opportunities: Regular Research Grants