Efficiency of Different Disinfectants on Bacillus cereus Sensu Stricto Biofilms on Stainless-Steel Surfaces in Contact With Milk

Author(s)

Silva, Higor Oliveira ^[1] ; Santos Lima, Joyce Aparecida ^[1] ; Gamero Aguilar, Carlos Eduardo ^[1] ; Marques Rossi, Gabriel Augusto ^[1] ; Mathias, Luis Antonio ^[1] ; Centola Vidal, Ana Maria ^[2]

Affiliation

^[1] Sao Paulo State Univ, Sch Agrarian & Veterinarian Sci, Dept Prevent Vet Med & Anim Reprod, Sao Paulo - Brazil

^[2] Univ Sao Paulo, Dept Vet Med, Sch Anim Sci & Food Engn, Sao Paulo - Brazil

• Document type: Journal article
• Source: FRONTIERS IN MICROBIOLOGY; v. 9, NOV 28 2018.
• Web of Science Citations: 0

Abstract

The species of the Bacillus cereus group have the ability to adhere to and form biofilms on solid surfaces, including stainless steel, a material widely used in food industries. Biofilms allow for recontamination during food processing, and the ``clean-in-place{''} (CIP) system is largely used by industries to control them. This study thus proposes to evaluate the efficacy of peracetic acid and sodium hypochlorite against biofilms induced on stainless-steel surfaces. The SAMN07414939 isolate (BioProject PRJNA390851), a recognized biofilm producer, was selected for biofilm induction on AISI 304 stainless steel. Biofilm induction was performed and classified into three categories: TCP (Tindalized, Contaminated, and Pasteurized milk), TCS (Tindalized milk Contaminated with Spores), and TCV (Tindalized milk Contaminated with Vegetative cells). Subsequently, the coupons were sanitized simulating a CIP procedure, on a pilot scale, using alkaline and acid solutions followed by disinfectants (peracetic acid and sodium hypochlorite). Microorganism adhesion on the surfaces reached 6.3 x 10(5) to 3.1 x 10(7) CFU/cm(-2). Results did not show significant differences (p > 0.05) for surface adhesion between the three tested categories (TOP, TCS, and TCV) or (p > 0.05) between the two disinfectants (peracetic acid and sodium hypochlorite). Microbial populations adhered to the stainless-steel coupons are equally reduced after treatment with peracetic acid and sodium hypochlorite, with no differences in the control of B. cereus s.s. biofilms on AISI 304 stainless-steel surfaces. (AU)

• FAPESP's process: 15/20874-0 - Bacillus cereus impact in dairy supply chain: gene expression related to gastroenteric toxins, spoilage enzymes during the shelf-life of pasteurized milk and determination of disinfectants efficacy on biofilms
• Grantee: Ana Maria Centola Vidal
• Support Opportunities: Regular Research Grants