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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Efficiency of Different Disinfectants on Bacillus cereus Sensu Stricto Biofilms on Stainless-Steel Surfaces in Contact With Milk

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Author(s):
Silva, Higor Oliveira [1] ; Santos Lima, Joyce Aparecida [1] ; Gamero Aguilar, Carlos Eduardo [1] ; Marques Rossi, Gabriel Augusto [1] ; Mathias, Luis Antonio [1] ; Centola Vidal, Ana Maria [2]
Total Authors: 6
Affiliation:
[1] Sao Paulo State Univ, Sch Agrarian & Veterinarian Sci, Dept Prevent Vet Med & Anim Reprod, Sao Paulo - Brazil
[2] Univ Sao Paulo, Dept Vet Med, Sch Anim Sci & Food Engn, Sao Paulo - Brazil
Total Affiliations: 2
Document type: Journal article
Source: FRONTIERS IN MICROBIOLOGY; v. 9, NOV 28 2018.
Web of Science Citations: 0
Abstract

The species of the Bacillus cereus group have the ability to adhere to and form biofilms on solid surfaces, including stainless steel, a material widely used in food industries. Biofilms allow for recontamination during food processing, and the ``clean-in-place{''} (CIP) system is largely used by industries to control them. This study thus proposes to evaluate the efficacy of peracetic acid and sodium hypochlorite against biofilms induced on stainless-steel surfaces. The SAMN07414939 isolate (BioProject PRJNA390851), a recognized biofilm producer, was selected for biofilm induction on AISI 304 stainless steel. Biofilm induction was performed and classified into three categories: TCP (Tindalized, Contaminated, and Pasteurized milk), TCS (Tindalized milk Contaminated with Spores), and TCV (Tindalized milk Contaminated with Vegetative cells). Subsequently, the coupons were sanitized simulating a CIP procedure, on a pilot scale, using alkaline and acid solutions followed by disinfectants (peracetic acid and sodium hypochlorite). Microorganism adhesion on the surfaces reached 6.3 x 10(5) to 3.1 x 10(7) CFU/cm(-2). Results did not show significant differences (p > 0.05) for surface adhesion between the three tested categories (TOP, TCS, and TCV) or (p > 0.05) between the two disinfectants (peracetic acid and sodium hypochlorite). Microbial populations adhered to the stainless-steel coupons are equally reduced after treatment with peracetic acid and sodium hypochlorite, with no differences in the control of B. cereus s.s. biofilms on AISI 304 stainless-steel surfaces. (AU)

FAPESP's process: 15/20874-0 - Bacillus cereus impact in dairy supply chain: gene expression related to gastroenteric toxins, spoilage enzymes during the shelf-life of pasteurized milk and determination of disinfectants efficacy on biofilms
Grantee:Ana Maria Centola Vidal
Support type: Regular Research Grants