Overexpression of ScMYBAS1 alternative splicing transcripts differentially impacts biomass accumulation and drought tolerance in rice transgenic plants

Author(s):Show less -

Peixoto-Junior, Rafael Favero ^[1] ; de Andrade, Larissa Mara ^[1] ; Brito, Michael dos Santos ^{[2, 3]} ; Nobile, Paula Macedo ^[2] ; Boer Martins, Alexandre Palma ^[1] ; Carlin, Samira Domingues ^[2] ; Ribeiro, Rafael Vasconcelos ^[4] ; de Souza Goldman, Maria Helena ^[5] ; Nebo Carlos de Oliveira, Joao Felipe ^[6] ; de Oliveira Figueira, Antonio Vargas ^[6] ; Creste, Silvana ^{[1, 2]}

Affiliation

^[1] Univ Sao Paulo, Fac Med Ribeirao Preto, Dept Genet, Ribeirao Preto, SP - Brazil

^[2] Inst Agron, Ctr Cana, Ribeirao Preto, SP - Brazil

^[3] Univ Fed Sao Paulo, Inst Ciencia & Tecnol, Dept Ciencia & Tecnol, Sao Jose Dos Campos - Brazil

^[4] Univ Estadual Campinas, Inst Biol, Dept Plant Biol, Campinas, SP - Brazil

^[5] Univ Sao Paulo, Fac Filosofia Ciencias & Letras Ribeirao Preto, Dept Biol, Ribeirao Preto, SP - Brazil

^[6] Univ Sao Paulo, Ctr Energia Nucl Agr, Piracicaba, SP - Brazil

• Document type: Journal article
• Source: PLoS One; v. 13, n. 12 DEC 5 2018.
• Web of Science Citations: 0

Abstract

Drought is the most significant environmental stress for agricultural production worldwide, and tremendous efforts have been made to improve crop yield under the increasing water scarcity. Transcription factors are major players in the regulation of water stress-related genes in plants. Recently, different MYB transcription factors were characterized for their involvement in drought response. A sugarcane R2R3-MYB gene (ScMYBAS1) and its four alternative forms of transcript (ScMYAS1-2, ScMYBAS1-3, ScMYBAS1-4 and ScMYBAS1-5) were identified in this study. The subcellular localization, in Nicotiniana benthamiana, of the TFs fused in frame with GFP revealed that ScMYBAS1-2-GFP and ScMYBAS1-3-GFP were observed in the nucleus. The overexpression of ScMYBAS1-2 and ScMYBAS1-3 spliced transcripts in rice promoted change in plant growth under both well-watered and drought conditions. The ScMYBAS1-2 and ScMYBAS1-3 transgenic lines revealed a higher relative water content (RWC) compared to the wild type before maximum stress under drought conditions. The ScMYBAS1-2 transgenic lines showed a reduction in biomass (total dry weight). Conversely, ScMYBAS1-3 showed an increased biomass (total dry weight) relative to the wild-type. The overexpression of ScMYBAS1-3 in rice transgenic lines showed involvement with drought tolerance and biomass and, for this reason, was considered a good target for plant transformation, particularly for use in developing genotypes with drought tolerance and biomass accumulation. (AU)

• FAPESP's process: 11/50661-8 - Characterization of sugar cane transcripts that respond to water stress in order to obtain drought-tolerant transgenic plants
• Grantee: Silvana Aparecida Creste Dias de Souza
• Support Opportunities: Program for Research on Bioenergy (BIOEN) - Regular Program Grants