The underlying mechanism of abiotic stress tolerance facilitated by the rol-genes (THrUSTER)

• Grant number: 19/08846-2
• Support Opportunities: Regular Research Grants
• Start date: September 01, 2019
• End date: August 31, 2021
• Field of knowledge: Biological Sciences - Genetics - Molecular Genetics and Genetics of Microorganisms
• Agreement: University of Copenhagen
• Mobility Program: SPRINT - Projetos de pesquisa - Mobilidade
• Principal Investigator: Ivan de Godoy Maia
• Grantee: Ivan de Godoy Maia
• Principal researcher abroad: Henrik VlK Lütken
• Institution abroad: University of Copenhagen, Copenhagen, Denmark
• Host Institution: Instituto de Biociências (IBB). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil
• Associated researchers: Bruno Trevenzoli Favero
• Associated research grant: 17/25139-2 - Mitochondrial dicarboxylate carriers and mitochondrial uncoupling proteins (UCP) as targets for improving plant tolerance to abiotic stress, AP.R

Abstract

Understanding how climate change is affecting plant growth is highly relevant given the potential to improve and increase crop production worldwide. The current collaborative SPRINT proposal will take advantage of already available Kalanchoë pinnata lines transformed with 35S::rolC and 35S::rolB (Danish partners) to investigate the impact of abiotic stresses, i.e. UV-B and drought, on growth and flavonoid accumulation in relation to untransformed plants (control).The hypothesis is that large-scale changes in gene expression are associated with rol overexpression and involve ROS-mediated signaling through MCF members.Therefore, diferente approaches will be employed to assess ROS and flavonoid accumulation as well as transcriptomic changes,and differentially expressed genes (DEG) in these plants. (AU)