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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Molecular characterization of Wnt pathway and function of -catenin overexpression in medulloblastoma cell lines

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Author(s):
Geron, Lenisa [1] ; Salomao, Karina Bezerra [2, 1] ; Borges, Kleiton Silva ; Andrade, Augusto Faria [1] ; Pereira Correa, Carolina Alves [1] ; Scrideli, Carlos Alberto [2] ; Tone, Luiz Gonzaga [2, 1]
Total Authors: 7
Affiliation:
[1] Univ Sao Paulo, Dept Genet, Ribeirao Preto Med Sch, 3900 Bandeirantes Ave, BR-14049900 Ribeirao Preto, SP - Brazil
[2] Univ Sao Paulo, Dept Pediat, Ribeirao Preto Med Sch, 3900 Bandeirantes Ave, BR-14049900 Ribeirao Preto, SP - Brazil
Total Affiliations: 2
Document type: Journal article
Source: Cytotechnology; v. 70, n. 6, p. 1713-1722, DEC 2018.
Web of Science Citations: 0
Abstract

Medulloblastoma (MB) is the most common malignant childhood brain tumor. MB is currently classified into four molecular subgroups (Wnt, Shh, Group 3, and Group 4). The wingless (Wnt) pathway is responsible for embryonic development and is deregulated in MB. We analyzed the activation of the Wnt pathway in MB cell lines and its correlation with the Shh pathway, with emphasis on the importance of cellular characterization. Transient -catenin transfection led to an increase in the -catenin gene and protein expression in MB cell lines. Wnt pathway activation resulted in a reduced number of colonies in all cell lines studied and a significant increase in the G2/M cell cycle phase only in ONS-76 cells. Regarding the Shh pathway, transfection caused a reduced expression of the PTCH1 and SMO genes only in the UW473 cells. Further studies are needed to understand the mechanism underlying the molecular events associated with the effects of Wnt activation in MB. (AU)

FAPESP's process: 14/07124-0 - Study of modulation of the Wnt pathway by Aurora kinases inhibitor AMG 900 in pediatric medulloblastoma cell lines
Grantee:Lenisa Geron
Support type: Scholarships in Brazil - Master
FAPESP's process: 14/20341-0 - Interactions between emerging therapeutic targets and developmental pathways associated with tumorigenesis: emphasis on pediatric malignancies
Grantee:Luiz Gonzaga Tone
Support type: Research Projects - Thematic Grants