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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

A novel cysteine-rich peptide regulates cell expansion in the tobacco pistil and influences its final size

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Brito, Michael S. [1, 2, 3] ; DePaoli, Henrique C. [1, 2, 4] ; Cossalter, Viviani [1, 5] ; Avanci, Nilton C. [1, 5] ; Ferreira, Pedro B. [1, 2] ; Azevedo, Mariana S. [6] ; Strini, Edward J. [1, 2] ; Quiapim, Andrea C. [1] ; Goldman, Gustavo H. [7] ; Peres, Lazar E. P. [8] ; Goldman, Maria Helena S. [1]
Total Authors: 11
[1] Univ Sao Paulo, Fac Filosofia Ciencias & Letras Ribeirao Preto, Dept Biol, Ave Bandeirantes 3900, BR-14040901 Ribeirao Preto, SP - Brazil
[2] Univ Sao Paulo, Fac Med Ribeirao Preto, PPG Genet, BR-14049900 Ribeirao Preto, SP - Brazil
[3] Univ Fed Sao Paulo, Inst Ciencia & Tecnol, Dept Ciencia & Tecnol, BR-12231280 Sao Jose Dos Campos, SP - Brazil
[4] US DOE, Joint Genome Inst, Walnut Creek, CA 94598 - USA
[5] Univ Sao Paulo, Fac Filosofia Ciencias & Letras Ribeirao Preto, PPG Biol Comparada, BR-14040901 Ribeirao Preto, SP - Brazil
[6] Inst Fed Parana, Campus Palmas, BR-85555000 Palmas, PR - Brazil
[7] Univ Sao Paulo, Fac Ciencias Farmaceut Ribeirao Preto, Dept Ciencias Farrnaceut, BR-14040903 Ribeirao Preto, SP - Brazil
[8] Univ Sao Paulo, Escola Super Agr Luiz de Queiroz, Dept Ciencias Biol, BR-13418900 Piracicaba, SP - Brazil
Total Affiliations: 8
Document type: Journal article
Source: Plant Science; v. 277, p. 55-67, DEC 2018.
Web of Science Citations: 0

Plant morphogenesis is dependent on cell proliferation and cell expansion, which are responsible for establishing final organ size and shape during development. Several genes have been described as encoding components of the plant cell development machinery, among which are the plant peptides. Here we describe a novel cysteine-rich plant peptide (68 amino acids), encoded by a small open reading frame gene (sORF). It is specifically expressed in the reproductive organs of Nicotiana tabacum and is developmentally regulated. N- and C-terminal translational fusions with GFP in protoplasts have demonstrated that the peptide is not secreted. Knockdown transgenic plants produced by RNAi exhibited enlarged pistils due to cell expansion and the gene was named Small Peptide Inhibitor of Cell Expansion (SPICE). Estimation of nuclear DNA content using flow cytometry has shown that cell expansion in pistils was not correlated with endoreduplication. Decreased SPICE expression also affected anther growth and pollen formation, resulting in male sterility in at least one transgenic plant. Our results revealed that SPICE is a novel reproductive organ specific gene that controls cell expansion, probably as a component of a signal transduction pathway. (AU)

FAPESP's process: 10/50213-2 - Study of Nicotiana tabacum pistil development and function: characterization of the stigma/style expressed genes and protein-protein interactions
Grantee:Maria Helena de Souza Goldman
Support type: Regular Research Grants