Low-level laser therapy modulates demyelination in mice

Author(s)

Nunes Duarte, Katherine Chuere ^{[1, 2]} ; Soares, Thais Torres ^{[1, 2]} ; Paiva Magri, Angela Maria ^{[1, 2]} ; Garcia, Livia Assis ^{[1, 2]} ; Le Sueur-Maluf, Luciana ^{[1, 2]} ; Muniz Renno, Ana Claudia ^{[1, 2, 3]} ; de Castro, Glaucia Monteiro ^{[1, 2]}

Affiliation

^[1] Univ Fed Sao Paulo, UNIFESP, Programa Interdisciplinar Ciencias Saude, Av Ana Costa 95, BR-11060001 Santos, SP - Brazil

^[2] Univ Fed Sao Paulo, UNIFESP, Dept Biociencias, Rua Silva Jardim 136, BR-11015020 Santos, SP - Brazil

^[3] Univ Fed Sao Paulo, UNIFESP, Programa Bioprod & Bioproc, Av Ana Costa 95, BR-11060001 Santos, SP - Brazil

• Document type: Journal article
• Source: JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY; v. 189, p. 55-65, DEC 2018.
• Web of Science Citations: 0

Abstract

There are no effective therapies for remyelination. Low-level laser therapy (LLLT) has been found advantageous in neurogenesis promotion, cell death prevention, and modulation of inflammation in central and peripheral nervous system models. The purpose of this study was to analyse LLLT effects on cuprizone-induced demyelination. Mice were randomly distributed into three groups: Control Laser (CTL), Cuprizone (CPZ), and Cuprizone Laser (CPZL). Mice from CPZ and CPZL groups were exposed to a 0.2% cuprizone oral diet for four complete weeks. Six sessions of transcranial laser irradiation were applied on three consecutive days, during the third and fourth weeks, with parameters of 36 J/cm(2), 50 mW, 0.028 cm(2) spot area, continuous wave, 1 J, 20 s, 1.78 W/cm(2) in a single point equidistant between the eyes and ears of CTL and CPZL mice. Motor coordination was assessed by the rotarod test. Twenty-four hours after the last laser session, all animals were euthanized, and brains were extracted. Serum was obtained for lactate dehydrogenase toxicity testing. Histomorphological analyses consisted of Luxol Fast Blue staining and immunohistochemistry. The results showed that laser-treated animals presented motor performance improvement, attenuation of demyelination, increased number of oligodendrocyte precursor cells, modulated microglial and astrocytes activation, and a milder toxicity by cuprizone. Although further studies are required, it is suggested that LLLT represents a feasible therapy for demyelinating diseases. (AU)

• FAPESP's process: 07/07828-3 - Characterization of the cuprizone-induced demyelization process in Lewis rat strain
• Grantee: Gláucia Monteiro de Castro
• Support Opportunities: Regular Research Grants