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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Molecular hydrogen potentiates hypothermia and prevents hypotension and fever in LPS-induced systemic inflammation

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Author(s):
Saramago, Eduardo A. [1] ; Borges, Gabriela S. [1] ; Singolani-, Jr., Carlitos G. [1] ; Nogueira, Jonatas E. [1, 2] ; Soriano, Renato N. [3] ; Carnio, Evelin C. [4] ; Branco, Luiz G. S. [5]
Total Authors: 7
Affiliation:
[1] Univ Sao Paulo, Med Sch Ribeirao Preto, BR-14049900 Sao Paulo - Brazil
[2] Univ Sao Paulo, Sch Phys Educ & Sports Ribeirao Preto, BR-14030680 Ribeirao Preto, SP - Brazil
[3] Univ Fed Juiz de Fora, Div Physiol & Biophys, Dept Basic Life Sci, BR-35010177 Governador Valadares, MG - Brazil
[4] Univ Sao Paulo, Nursing Sch Ribeirao Preto, BR-14040902 Sao Paulo - Brazil
[5] Univ Sao Paulo, Dent Sch Ribeirao Preto, BR-14040904 Sao Paulo - Brazil
Total Affiliations: 5
Document type: Journal article
Source: BRAIN BEHAVIOR AND IMMUNITY; v. 75, p. 119-128, JAN 2019.
Web of Science Citations: 6
Abstract

Molecular hydrogen (H-2) exerts anti-oxidative, anti-apoptotic, and anti-inflammatory effects. Here we tested the hypothesis that H-2 modulates cardiovascular, inflammatory, and thermoregulatory changes in systemic inflammation (SI) induced by lipopolysaccharide (LPS) at different doses (0.1 or 1.5 mg/kg, intravenously, to induce mild or severe SI) in male Wistar rats (250-300 g). LPS or saline was injected immediately before the beginning of 360-minute inhalation of H-2 (2% H-2, 21% O-2, balanced with nitrogen) or room air (21% O-2, balanced with nitrogen). Deep body temperature (Tb) was measured by dataloggers pre-implanted in the peritoneal cavity. H-2 caused no change in cardiovascular, inflammatory parameters, and Tb of control rats (treated with saline). During mild SI, H-2 reduced plasma surges of proinflammatory cytokines (TNF-alpha and IL-6) while caused an increase in plasma IL-10 (anti-inflammatory cytokine) and prevented fever. During severe SI, H-2 potentiated hypothermia, and prevented fever and hypotension, which coincided with reduced plasma nitric oxide (NO) production. Moreover, H-2 caused a reduction in surges of proinflammatory cytokines (plasma TNF-alpha and IL-1 beta) and prostaglandin E-2 {[}(PGE(2)), in plasma and hypothalamus], and an increase in plasma IL-10. These data are consistent with the notion that H-2 blunts fever in mild SI, and during severe SI potentiates hypothermia, prevents hypotension reducing plasma NO production, and exerts anti-inflammatory effects strong enough to prevent fever by altering febrigenic signaling and ultimately down-modulating hypothalamic PGE(2) production. (AU)

FAPESP's process: 16/17681-9 - Pathophysiological changes during systemic inflammation
Grantee:Luiz Guilherme de Siqueira Branco
Support Opportunities: Research Projects - Thematic Grants