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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Quantitation of pulmonary fungal burden in Paracoccidioides brasiliensis-infected mice by real-time PCR

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Author(s):
Costa, Marcelo Vieira [1] ; Landgraf, Taise Natali [2] ; Correa, Priscila C. [2] ; Lemos Souza, Igor Emiliano [2] ; Fernandes, Fabricio Freitas [3] ; Panunto-Castelo, Ademilson [1]
Total Authors: 6
Affiliation:
[1] Univ Sao Paulo, Fac Filosofia Ciencias & Letras Ribeirao Preto, Dept Biol, Av Bandeirantes 3900, BR-14040901 Ribeirao Preto, SP - Brazil
[2] Univ Sao Paulo, Fac Med Ribeirao Preto, Programa Posgrad Imunol Basica & Aplicada, Ribeirao Preto, SP - Brazil
[3] Univ Sao Paulo, Fac Med Ribeirao Preto, Dept Biol Celular & Mol, Ribeirao Preto, SP - Brazil
Total Affiliations: 3
Document type: Journal article
Source: Revista do Instituto de Medicina Tropical de São Paulo; v. 61, 2019.
Web of Science Citations: 1
Abstract

ABSTRACT Although colony-forming unit (CFU) counting is widely used to quantify fungal load in tissue from animal experimentally infected with Paracoccidioides brasiliensis, several technical disadvantages have been described. Here we developed highly accurate quantitative PCR (qPCR) assays to determine the relative P brasiliensis load in lungs from infected mice. SYBR Green- and TaqMan-based assays using primers and probe for the 43-kDa glycoprotein (gp43) gene detected as little as 270 gene copies (about 2 fg of DNA) per reaction. Although qPCR assays cannot distinguish between living and dead yeasts, we found a highly positive linear correlation between CFU and qPCR. (AU)

FAPESP's process: 13/12278-3 - Screening of Paracoccidioides brasiliensis antigens with potential immunotherapeutic application in paracoccidioidomicose
Grantee:Ademilson Panunto-Castelo
Support Opportunities: Regular Research Grants
FAPESP's process: 17/01390-8 - Evaluation of plasmid DNA vaccine containing the gene of 60kDa heat shock protein from Paracoccidioides brasiliensis as therapy against experimental paracoccidiodomycosis in mice
Grantee:Ademilson Panunto-Castelo
Support Opportunities: Regular Research Grants