| Full text | |
| Author(s): Show less - |
Muniz, Bruno Vilela
[1]
;
Baratelli, Diego
[2]
;
Di Carla, Stephany
[1]
;
Serpe, Luciano
[1]
;
da Silva, Camila Batista
[1]
;
Guilherme, Viviane Aparecida
[3]
;
de Morais Ribeiro, Ligia Nunes
[3]
;
Saia Cereda, Cintia Maria
[3]
;
de Paula, Eneida
[3]
;
Volpato, Maria Cristina
[1]
;
Groppo, Francisco Carlos
[1]
;
Fraceto, Leonardo Fernandes
[2]
;
Franz-Montan, Michelle
[1]
Total Authors: 13
|
| Affiliation: | [1] Univ Campinas UNICAMP, Piracicaba Dent Sch, Dept Physiol Sci, Piracicaba, SP - Brazil
[2] Sao Paulo State Univ UNESP, Dept Environm Engn, Inst Sci & Technol Sorocaba, Sorocaba, SP - Brazil
[3] Univ Campinas Unicamp, Dept Biochem & Tissue Biol, Inst Biol, Campinas, SP - Brazil
Total Affiliations: 3
|
| Document type: | Journal article |
| Source: | SCIENTIFIC REPORTS; v. 8, DEC 19 2018. |
| Web of Science Citations: | 2 |
| Abstract | |
This study reports the development of nanostructured hydrogels for the sustained release of the eutectic mixture of lidocaine and prilocaine (both at 2.5%) for intraoral topical use. The local anesthetics, free or encapsulated in poly(e-caprolactone) nanocapsules, were incorporated into CARBOPOL hydrogel. The nanoparticle suspensions were characterized in vitro in terms of particle size, polydispersity, and surface charge, using dynamic light scattering measurements. The nanoparticle concentrations were determined by nanoparticle tracking analysis. Evaluation was made of physicochemical stability, structural features, encapsulation efficiency, and in vitro release kinetics. The CARBOPOL hydrogels were submitted to rheological, accelerated stability, and in vitro release tests, as well as determination of mechanical and mucoadhesive properties, in vitro cytotoxicity towards FGH and HaCaT cells, and in vitro permeation across buccal and palatal mucosa. Anesthetic efficacy was evaluated using Wistar rats. Nanocapsules were successfully developed that presented desirable physicochemical properties and a sustained release profile. The hydrogel formulations were stable for up to 6 months under critical conditions and exhibited non-Newtonian pseudoplastic flows, satisfactory mucoadhesive strength, non-cytotoxicity, and slow permeation across oral mucosa. In vivo assays revealed higher anesthetic efficacy in tail-flick tests, compared to a commercially available product. In conclusion, the proposed hydrogel has potential for provision of effective and longer-lasting superficial anesthesia at oral mucosa during medical and dental procedures. These results open perspectives for future clinical trials. (AU) | |
| FAPESP's process: | 12/06974-4 - Development, biocompatibility and permeation studies on gel formulations of poly-epsilon-caprolactone nanocapsules containing local anesthetics |
| Grantee: | Michelle Franz Montan Braga Leite |
| Support Opportunities: | Research Grants - Young Investigators Grants |
| FAPESP's process: | 13/22326-5 - Development, biocompatibility and permeation studies on gel formulations of poly-epsilon-caprolactone nanocapsules containing local anesthetics. |
| Grantee: | Michelle Franz Montan Braga Leite |
| Support Opportunities: | Scholarships in Brazil - Young Researchers |
| FAPESP's process: | 14/14457-5 - Lipid-based nanocarriers (SLN/NLC and remote-loading liposomes) used to improve the upload and potency of local anesthetics |
| Grantee: | Eneida de Paula |
| Support Opportunities: | Research Projects - Thematic Grants |