NMR insights on nano silver post-surgical treatment of superficial caseous lymphadenitis in small ruminants

Author(s):Show less -

Stanisic, Danijela ^[1] ; Fregonesi, Natalia L. ^[1] ; Barros, Caio H. N. ^[1] ; Pontes, Joao G. M. ^[1] ; Fulaz, Stephanie ^[1] ; Menezes, Ulisses J. ^[2] ; Nicoleti, Jorge L. ^[2] ; Castro, Thiago L. P. ^[3] ; Seyffert, Nubia ^[4] ; Azevedo, Vasco ^[3] ; Duran, Nelson ^{[5, 6]} ; Portela, Ricardo W. ^[2] ; Tasic, Ljubica ^{[5, 1]}

Affiliation

^[1] Univ Estadual Campinas, Inst Quim, Dept Quim Organ, Lab Quim Biol, Campinas, SP - Brazil

^[2] Univ Fed Bahia, Inst Ciencias Saude, Lab Imunol & Biol Mol, Salvador, BA - Brazil

^[3] Univ Fed Minas Gerais, Inst Ciencias Biol, Dept Biol Geral, Belo Horizonte, MG - Brazil

^[4] Univ Fed Bahia, Inst Biol, Lab Bacteriol & Saude, Salvador, BA - Brazil

^[5] Univ Estadual Campinas, NanoBioss Inst Chem, Campinas, SP - Brazil

^[6] UFABC, Sao Paulo, SP - Brazil

• Document type: Journal article
• Source: RSC ADVANCES; v. 8, n. 71, p. 40778-40786, 2018.
• Web of Science Citations: 1

Abstract

Caseous lymphadenitis (CL), caused by a pathogen of the second class of biosafety - Corynebacterium pseudotuberculosis, is a chronic and severe infectious disease that affects small ruminants and requires long, ineffective treatment which generally leads to animal sacrifice so as to stop the disease spreading. The infected animals suffer the excision of affected superficial lymph nodes and post-surgical treatment with iodine (10% solution in ethanol) and, sometimes, prolonged antibiotic use, but only if the sick animals are of great importance to breeding. Herein, we propose a cheap and easy to apply treatment of CL with excellent results using biogenic silver nanoparticles (AgNP) based technology. AgNP antibacterial properties were investigated in vitro against Corynebacterium pseudotuberculosis cells and in vivo on small ruminants with CL. Treatment of surgical wounds resulting from the excision of superficial CL lesions with a AgNP-based cream was compared to the standard post-surgical treatment method by iodine. Also, the effects of AgNP-based cream treatment were evaluated and compared with the effects of the iodine CL treatment by serum NMR-based metabolomics. Serum samples were collected from 29 animals, 9 sheep and 20 goats, during the treatments and analyzed. All animals showed stable serum metabolomes when iodine or AgNP-based cream effects were compared. The AgNP-based cream treatment showed excellent results, especially in accelerating the healing of wounds, which occurred two to three times faster in comparison with the iodine treatment. AgNP-based cream treatment also prevented CL reappearance and did not cause any side effects on animals. This is the first report on very effective post-surgical treatment of superficial CL in small ruminants based on biogenic silver nanoparticles, which might open up the possibility for a safe veterinary application of AgNP-based cream. (AU)

• FAPESP's process: 15/12534-5 - Study on the supramolecular interactions in Silver Nanoparticles and their antimicrobial activity
• Grantee: Caio Henrique Nasi de Barros
• Support Opportunities: Scholarships in Brazil - Master
• FAPESP's process: 14/18938-8 - Metabolomic profiling of severe mental disorders using Nuclear Magnetic Ressonance (NMR)
• Grantee: Ljubica Tasic
• Support Opportunities: Regular Research Grants
• FAPESP's process: 18/06510-4 - Application of Nuclear Magnetic Resonance (NMR) in metabolomics and metabonomics of human diseases
• Grantee: Ljubica Tasic
• Support Opportunities: Regular Research Grants