A new chemotherapeutic approach using doxorubicin simultaneously with geopropolis favoring monocyte functions

Author(s)

Garcia Oliveira, Lucas Pires ^[1] ; Conte, Fernanda Lopes ^[1] ; Cardoso, Eliza de Oliveira ^[1] ; Conti, Bruno Jose ^[1] ; Santiago, Karina Basso ^[1] ; Golim, Marjorie de Assis ^[2] ; Feltran, Georgia da Silva ^[1] ; Zambuzzi, Willian Fernando ^[1] ; Sforcin, Jose Mauricio ^[1]

Affiliation

^[1] Sao Paulo State Univ UNESP, Inst Biosci, Botucatu, SP - Brazil

^[2] UNESP, Sch Med, Botucatu Blood Ctr, BR-18618970 Botucatu, SP - Brazil

• Document type: Journal article
• Source: Life Sciences; v. 217, p. 81-90, JAN 15 2019.
• Web of Science Citations: 1

Abstract

Aims: Chemotherapy has been widely used to treat cancer although it may affect non-target cells involved in the immune response. This work aimed at elucidating whether the chemotherapeutic agent doxorubicin in combination with geopropolis produced by Melipona fasciculata Smith could affect nontumor immune cells, evaluating their immunomodulatory effects on human monocytes. Main methods: Cell viability, expression of cell markers (HLA-DR, TLR-2, TLR-4, C80 and CD40), cytokine production (TNF-alpha, IL-1 beta, IL-6 and IL-10), intracellular pathways (NF-kappa B and autophagy), the microbicidal activity of monocytes and hydrogen peroxide (H2O2) production were analyzed. Key findings: Data showed that doxorubicin + geopropolis diminished IL-6 secretion, stimulated TNF-a and IL-10 production, TLR-4 and CD80 expression, NF-kappa B and autophagy pathway, as well as the bactericidal activity. (AU)

• FAPESP's process: 16/02249-4 - Immunotoxicologic / immunomodulatory action of geopropolis associated with doxorubicin on human monocytes
• Grantee: Lucas Pires Garcia Oliveira
• Support Opportunities: Scholarships in Brazil - Master