At Term, XmO and XpO Mouse Placentas Show Differences in Glucose Metabolism in the Trophectoderm-Derived Outer Zone

Author(s)

He, Nannan ^[1] ; Lim, Shujing J. ^{[1, 2]} ; Moreira de Mello, Joana C. ^[3] ; Navarro, Injerreau ^[1] ; Bialecka, Monika ^[1] ; Salvatori, Daniela C. F. ^{[1, 4]} ; van der Westerlaken, Lucette A. J. ^[5] ; Pereira, Lygia V. ^[3] ; Lopes, Susana M. Chuva de Sousa ^{[1, 6]}

Affiliation

^[1] Leiden Univ, Med Ctr, Dept Anat & Embryol, Leiden - Netherlands

^[2] Univ Edinburgh, Coll Med & Vet Med, Edinburgh, Midlothian - Scotland

^[3] Univ Sao Paulo, Dept Genet & Evolutionary Biol, Sao Paulo - Brazil

^[4] Leiden Univ, Med Ctr, Cent Lab Anim Facil, Leiden - Netherlands

^[5] Leiden Univ, Med Ctr, Dept Gynaecol, Leiden - Netherlands

^[6] Ghent Univ Hosp, Dept Reprod Med, Ghent - Belgium

• Document type: Journal article
• Source: FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY; v. 5, 2017.
• Web of Science Citations: 1

Abstract

Genetic mouse model (39,XO) for human Turner Syndrome (45,XO) harboring either a single maternally inherited (Xm) or paternally inherited (Xp) chromosome show a pronounced difference in survival rate at term. However, a detailed comparison of XmO and XpO placentas to explain this difference is lacking. We aimed to investigate the morphological and molecular differences between XmO and XpO term mouse placentas. We observed that XpO placentas at term contained a significantly larger area of glycogen cells (GCs) in their outer zone, compared to XmO, XX, and XY placentas. In addition, the outer zone of XpO placentas showed higher expression levels of lactate dehydrogenase (Ldha) than XmO, XX, and XY placentas, suggestive of increased anaerobic glycolysis. In the labyrinth, we detected significantly lower expression level of trophectoderm (TE)-marker keratin 19 (Krt19) in XpO placentas than in XX placentas. The expression of other TE-markers was comparable as well as the area of TE-derived cells between XO and wild-type labyrinths. XpO placentas exhibited specific defects in the amount of GCs and glucose metabolism in the outer zone, suggestive of increased anaerobic glycolysis, as a consequence of having inherited a single Xp chromosome. In conclusion, the XpO genotype results in a more severe placental phenotype at term, with distinct abnormalities regarding glucose metabolism in the outer zone. (AU)

• FAPESP's process: 09/17481-6 - X-chromosome inactivation in humans: initiation and imprinting
• Grantee: Joana Carvalho Moreira de Mello
• Support type: Scholarships in Brazil - Doctorate
• FAPESP's process: 13/08135-2 - CTC - Center for Cell-Based Therapy
• Grantee: Dimas Tadeu Covas
• Support type: Research Grants - Research, Innovation and Dissemination Centers - RIDC
• FAPESP's process: 15/03610-0 - In silico analysis of the X-chromosome epigenetic state in human pre-implantation embryos
• Grantee: Joana Carvalho Moreira de Mello
• Support type: Scholarships in Brazil - Post-Doctorate