| Full text | |
| Author(s): |
João Luiz de Oliveira Madeira
[1]
;
Luciane Zgoda Bussmann
[2]
;
Helena Panteliou Lima-Valassi
[3]
;
Berenice Bilharinho de Mendonça
[4]
Total Authors: 4
|
| Affiliation: | [1] Universidade de São Paulo. Faculdade de Medicina. Hospital das Clínicas - Brasil
[2] Universidade de São Paulo. Faculdade de Medicina. Hospital das Clínicas - Brasil
[3] Universidade de São Paulo. Faculdade de Medicina. Hospital das Clínicas - Brasil
[4] Universidade de São Paulo. Faculdade de Medicina. Hospital das Clínicas - Brasil
Total Affiliations: 4
|
| Document type: | Journal article |
| Source: | Arquivos Brasileiros de Endocrinologia e Metabologia; v. 58, n. 3, p. 232-236, 2014-04-00. |
| Abstract | |
Objective : Our aim was to correlate 11-deoxycortisol levels obtained by two currently available techniques for 11-deoxycortisol measurement: radioimmunoassay, and high performance liquid chromatography followed by tandem mass spectrometry (MS/MS). The latter is the gold standard method for steroid hormone measurement. Materials and methods : We selected 88 samples and the results of these two methods were compared by Deming regression. Results : The analytical sensitivity of the RIA was 0.30 ng/mL, with inadequate linearity and inadequate precision profile (34% of the samples had a CV ≥ 20%). From the selected samples, 54 had measurable levels of 11-deoxycortisol in both methods and were used in the comparison. The comparison of RIA with LC-MS/MS showed an overestimation of the results by RIA. The correlation coefficient was 0.610; linear regression slope was 3.751; and the intercept was 0.145, indicating a poor correlation between the two methods. Conclusion : We concluded that 11-deoxycortisol measured by radioimmunoassay, despite a good analytical sensitivity, showed very low specificity, precluding its use as a reliable method for 11-deoxycortisol measurement. Arq Bras Endocrinol Metab. 2014;58(3):232-6 (AU) | |
| FAPESP's process: | 10/05188-0 - Validation of a radioimmunoassay for 11-deoxycortisol measurement for the diagnosis of hyperandrogenic disorders |
| Grantee: | João Luiz de Oliveira Madeira |
| Support Opportunities: | Scholarships in Brazil - Scientific Initiation |