Padronização da técnica imunoenzimática do ELISA de captura, no sistema avidina-biotina para a identificação de sangue ingerido por Lutzomyia (Lutzomyia) longipalpis (Lutz & Neiva, 1912)

Ana Maria Marassá; Cleide Aschenbrenner Consales; Eunice Aparecida Bianchi Galati

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(Reference retrieved automatically from SciELO through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Enzyme-linked Immunosorbent Assay biotin/avidin method standardization, for identification of Lutzomyia (Lutzomyia) longipalpis bloodmeals (Lutz & Neiva, 1912)

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Author(s):	Ana Maria Marassá ^[1] ; Cleide Aschenbrenner Consales ^[2] ; Eunice Aparecida Bianchi Galati ^[3] Total Authors: 3
Affiliation:	^[1] Instituto Adolfo Lutz ^[2] Instituto Pasteur ^[3] Universidade de São Paulo. Faculdade de Saúde Pública. Departamento de Epidemiologia Total Affiliations: 3
Document type:	Journal article
Source:	Revista da Sociedade Brasileira de Medicina Tropical; v. 37, n. 6, p. 441-446, 2004-12-00.
Abstract
Bloodmeals taken by insects constitute an important parameter for clarifying aspects of the transmission of zoonoses, including leishmaniases. Immunological assays can be used to investigate the attraction of vectors to animals, which may be hosts of these parasitoses. The objective of this study was to standardize a sandwich enzyme-linked immunosorbent assay and titer samples with different time periods of digestion, in laboratory-bred Lutzomyia longipalpis fed on rats. In the light of the high sensitivity that the biotin-avidin method permits, the technique provided at least ninety repeat tests for each sample and identified recent bloodmeals taken by these insects. Bloodmeals were detectable up to 12 and 24h after blood ingestion, and a significant difference between these titers was observed. (AU)

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