Development of a method for quantitative determination of the cytotoxic agent piplartine (piperlongumine) in multiple skin layers

Author(s)

Carvalho, Vanessa F. M. ^[1] ; Giacone, Daniela V. ^[1] ; Costa-Lotufo, Leticia V. ^[1] ; Silveira, Edilberto Rocha ^[2] ; Lopes, Luciana B. ^[1]

Affiliation

^[1] Univ Sao Paulo, Inst Biomed Sci, Dept Pharmacol, Av Prof Lineu Prestes 1524, Sao Paulo, SP - Brazil

^[2] Univ Fed Ceara, Dept Inorgan & Organ Chem, Fortaleza, Ceara - Brazil

• Document type: Journal article
• Source: BIOMEDICAL CHROMATOGRAPHY; v. 33, n. 2 FEB 2019.
• Web of Science Citations: 1

Abstract

This study reports the development of a simple and reproducible method, with high rates of recovery, to extract the cytotoxic agent piplartine from skin layers, and a sensitive and rapid UV-HPLC method for its quantification. Considering the potential of piplartine for topical treatment of skin cancer, this method may find application for formulation development and pharmacokinetics studies to assess cutaneous bioavailability. Porcine skin was employed as a model for human tissue. Piplartine was extracted from the stratum corneum (SC) and remaining viable skin layers (VS) using methanol, vortex homogenization and bath sonication, and subsequently assayed by HPLC using a C-18 column, and 1:1 (v/v) acetonitrile-water (adjusted to pH 4.0 with acetic acid 0.1%) as mobile phase. The quantification limit of piplartine was 0.2 mu g/mL (0.6 mu m), and the assay was linear up to 5 mu g/mL (15.8 mu m), with within-day and between-days assay coefficients of variation and relative errors <15%. Piplartine recovery from SC and VS varied from 86 to 96%. The method was suitable to assay samples from skin penetration studies, enabling detection of differences in cutaneous delivery in different skin compartments resulting from treatment with various formulations and time periods. (AU)

• FAPESP's process: 13/16617-7 - Nanostructured systems for topical delivery and co-localization of chemopreventive and chemoterapeutic agents in the skin and breast tissue
• Grantee: Luciana Biagini Lopes
• Support Opportunities: Research Grants - Young Investigators Grants
• FAPESP's process: 17/07856-9 - Cationic nanoemulsions for piplartine cutaneous delivery and treatment of skin tumors
• Grantee: Daniela Veronesi Giacone
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• FAPESP's process: 17/04174-4 - Development and evaluation of cationic bioadhesive nanocarriers as a platform to localize piplartine in the breast for tumor treatment
• Grantee: Vanessa Franco Carvalho Dartora
• Support Opportunities: Scholarships in Brazil - Doctorate