Cationic nanoemulsions for piplartine cutaneous delivery and treatment of skin tumors

• Grant number: 17/07856-9
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Effective date (Start): July 01, 2017
• Effective date (End): December 31, 2017
• Field of knowledge: Health Sciences - Pharmacy - Pharmaceutical Technology
• Principal Investigator: Luciana Biagini Lopes
• Grantee: Daniela Veronesi Giacone
• Host Institution: Instituto de Ciências Biomédicas (ICB). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Associated research grant: 13/16617-7 - Nanostructured systems for topical delivery and co-localization of chemopreventive and chemoterapeutic agents in the skin and breast tissue, AP.JP

Abstract

In this project, we propose to evaluate the ability of cationic nanoemulsions to promote cutaneous penetration and localization of the cytotoxic compound piplartine as a novel treatment option for tumors affecting the skin. The high incidence of skin tumors, the need of self-administered topical treatment options for patients for whom the surgery is not recommended, and the absence of topical formulations targeting the cutaneous localization of therapeutics agents for maximizing efficacy justify our proposal. Piplartine is an alkaloid / amide component of Piper species that has significant cytotoxic and antiproliferative activity in several tumor cell lines. As nanocarrier, we propose the use of oil-in-water cationic nanoemulsions with bioadhesive properties in order to increase the retention and the formulation interaction with the tissue. The formulations will be selected according to particle size, charge and rheological characteristics. Then, how these parameters influence the stability will be studied, as well as cutaneous permeability and the transport and localization of piplartine in/through the skin. The formulations cytotoxic effects will be evaluated in cutaneous equivalents of skin cancer. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

GIACONE, V, DANIELA; DARTORA, VANESSA F. M. C.; DE MATOS, JENYFFER K. R.; PASSOS, JULIA S.; MIRANDA, DANIEL A. G.; DE OLIVEIRA, ERICA A.; SILVEIRA, EDILBERTO R.; COSTA-LOTUFO, V, LETICIA; MARIA-ENGLER, SILVYA S.; LOPES, LUCIANA B.. Effect of nanoemulsion modification with chitosan and sodium alginate on the topical delivery and efficacy of the cytotoxic agent piplartine in 2D and 3D skin cancer models. International Journal of Biological Macromolecules, v. 165, n. A, p. 1055-1065, DEC 15 2020. (17/07856-9, 15/17177-6, 16/16554-3, 17/04174-4, 20/01208-8, 18/18813-1, 18/13877-1)

CARVALHO, VANESSA F. M.; GIACONE, DANIELA V.; COSTA-LOTUFO, LETICIA V.; SILVEIRA, EDILBERTO ROCHA; LOPES, LUCIANA B.. Development of a method for quantitative determination of the cytotoxic agent piplartine (piperlongumine) in multiple skin layers. BIOMEDICAL CHROMATOGRAPHY, v. 33, n. 2, FEB 2019. (13/16617-7, 17/07856-9, 17/04174-4)

GIACONE, DANIELA, V; DARTORA, VANESSA F. M. C.; DE MATOS, JENYFFER K. R.; PASSOS, JULIA S.; MIRANDA, DANIEL A. G.; DE OLIVEIRA, ERICA A.; SILVEIRA, EDILBERTO R.; COSTA-LOTUFO, LETICIA, V; MARIA-ENGLER, SILVYA S.; LOPES, LUCIANA B.. Effect of nanoemulsion modification with chitosan and sodium alginate on the topical delivery and efficacy of the cytotoxic agent piplartine in 2D and 3D skin cancer models. International Journal of Biological Macromolecules, v. 165, p. 11-pg., 2020-12-15. (18/13877-1, 18/18813-1, 17/07856-9, 17/04174-4, 20/01208-8, 15/17177-6, 16/16554-3)