Advanced search
Start date
Betweenand
Related content
(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Hyperbaric oxygen affects endothelial progenitor cells proliferation in vitro

Full text
Author(s):
Benincasa, Julia C. [1] ; de Freitas Filho, Luiz H. [1] ; Carneiro, Giane D. [1] ; Sielski, Micheli S. [1] ; Giorgio, Selma [2] ; Werneck, Claudio C. [3] ; Vicente, Cristina P. [1]
Total Authors: 7
Affiliation:
[1] State Univ Campinas UNICAMP, Dept Struct & Funct Biol, Sao Paulo - Brazil
[2] Univ Estadual Campinas, Dept Anim Biol, BR-13083865 Campinas - Brazil
[3] State Univ Campinas UNICAMP, Inst Biol, Dept Biochem & Tissue Biol, Sao Paulo - Brazil
Total Affiliations: 3
Document type: Journal article
Source: Cell Biology International; v. 43, n. 2, p. 136-146, FEB 2019.
Web of Science Citations: 3
Abstract

Hyperbaric oxygen is a clinical treatment that contributes to wound healing by increasing fibroblasts proliferation, collagen synthesis, and production of growth factors, inducing angiogenesis and inhibiting antimicrobial activity. It also has been shown that hyperbaric oxygen treatment (HBO), through the activation of nitric oxide synthase promotes an increase in the nitric oxide levels that may improve endothelial progenitor cells (EPC) mobilization from bone marrow to the peripheral blood and stimulates the vessel healing process. However, cellular mechanisms involved in cell proliferation and activation of EPC after HBO treatment remain unknown. Therefore, the present work aimed to analyze the effect of HBO on the proliferation of pre-treated bone marrow-derived EPC with TNF-alpha. Also, we investigated the expression of ICAM and eNOS by immunochemistry, the production of reactive species of oxygen and performed an in vitro wound healing. Although 1h of HBO treatment did not alter the rate of in vitro wound closure or cell proliferation, it increased eNOS expression and decreased ICAM expression and reactive oxygen species production in cells pre-treated with TNF-alpha. These results indicate that HBO can decrease the inflammatory response in endothelial cells mediated by TNF-alpha, and thus, promote vascular recovery after injury. (AU)

FAPESP's process: 15/23767-0 - Development of biomarkers and Leishmania infantum strains
Grantee:Selma Giorgio
Support Opportunities: Regular Research Grants