| Full text | |
| Author(s): |
Belardin, Larissa Berloffa
[1]
;
Antoniassi, Mariana Pereira
[1]
;
Camargo, Mariana
[1]
;
Intasqui, Paula
[1]
;
Fraietta, Renato
[1]
;
Bertolla, Ricardo Pimenta
[2, 1]
Total Authors: 6
|
| Affiliation: | [1] Univ Fed Sao Paulo, Div Urol, Dept Surg, Sao Paulo - Brazil
[2] Hosp Sao Paulo, Sao Paulo - Brazil
Total Affiliations: 2
|
| Document type: | Journal article |
| Source: | SCIENTIFIC REPORTS; v. 9, JAN 29 2019. |
| Web of Science Citations: | 4 |
| Abstract | |
Matrix Metalloproteinases (MMPs) and their regulators - Tissue Inhibitors of Matrix Metalloproteinases (TIMPs) - participate in extracellular matrix remodeling, fibrosis, and semen liquefaction, as well as to inflammatory activity. Seminal plasma has been shown to contain MMPs (MMP-2 and MMP-9) and TIMPs (TIMP-1 and TIMP-2). Also, a link between MMPs gene expression and excessive reactive oxygen species (ROS) has been established. In semen, ROS are associated with altered sperm function and increased DNA fragmentation. In this study, it is hypothesized that seminal MMPs and TIMPs levels are associated with sperm DNA fragmentation due to the fact that MMPs have been associated with semen quality. We also hypothesized that these proteins could predict DNA fragmentation status in sperm. Therefore, this study set out to verify if sperm DNA fragmentation levels relate to seminal levels of members of the MMP and TIMP protein families. The High sperm DNA fragmentation group presented lower seminal plasma levels of MMP-2, MMP-7, TIMP-1, TIMP-2 and TIMP-4 when compared to Low sperm DNA fragmentation group. Also, samples in the high sperm DNA fragmentation group presented higher acrosome integrity and lower mitochondrial activity levels when compared to low sperm DNA fragmentation samples. In the logistic regression analysis, MMP-2, MMP-7, and TIMP-4 classified samples as low and high sperm DNA fragmentation, with an overall model fit of 74.5%. Results from this study may demonstrate a specific inflammatory mechanism in samples with high sperm DNA fragmentation. This, in turn, can lead to the development of new studies regarding this mechanism and, in the future, create an opportunity to treat these patients for sperm DNA fragmentation by treating inflammatory seminal activity. (AU) | |
| FAPESP's process: | 14/17185-6 - Confirmatory proteomic analysis of biomarkers of sperm functional alteration and seminal oxidative stress |
| Grantee: | Ricardo Pimenta Bertolla |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 16/05487-3 - Epididymal regulation of fertility in men. |
| Grantee: | Larissa Berloffa Belardin |
| Support Opportunities: | Scholarships in Brazil - Doctorate |