Apoptosis of CD5+ cells and lymphocyte proliferation in bovine leukemia virus-infected dairy cows

Author(s)

F.N Souza ^[1] ; A.O Latorre ^[2] ; B.D Caniceiro ^[3] ; M Sakai ^[4] ; K Kieling ^[5] ; M.G Blagitz ^[6] ; A.M.M.P Della Libera ^[7]

Affiliation

^[1] Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia. Departamento de Clínica Médica

^[2] Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia. Departamento de Patologia

^[3] Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia. Departamento de Patologia

^[4] Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia. Departamento de Patologia

^[5] Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia. Departamento de Patologia

^[6] Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia. Departamento de Clínica Médica

^[7] Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia. Departamento de Clínica Médica

• Document type: Journal article
• Source: Arquivo Brasileiro de Medicina Veterinária e Zootecnia; v. 63, n. 5, p. 1124-1130, 2011-10-00.

Abstract

The purpose of the present trail was to evaluate the lymphocyte proliferation and the apoptosis rates of CD5+ cells in dairy cows infected with bovine leukemia virus (BLV) with distinct lymphocyte profiles in infected animals known as alymphocytotic (AL) and persistent lymphocytosis (PL). A total of 100 Holstein cows were sera tested for bovine leukemia virus through agar gel immunodiffusion (AGID) and enzyme-linked immunosorbent-assay (ELISA). From these animals, 15 cows were selected and divided uniformly in 3 groups (negative, AL, LP). The lymphocyte proliferation was performed using flow cytometric measurement of CFSE-DA dye, where 2x10(6)/mL lymphocytes were plated per well. The apoptosis of CD5+ cells from peripheral blood was performed using the annexin V-FITC to measure the apoptosis rates and the identification of CD5+ was accessed using monoclonal antibodies. Animals from the LP group showed lower lymphocyte proliferation and also lower apoptosis rates of CD5+ cells compared with negative and AL animals. The development of PL which resulted from an increase in B cell count, is due to the decrease in the apoptosis rates of CD5+ cells, and the higher lymphocyte proliferation appears to be limited only in the initial stages of development of LP. (AU)