| Full text | |
| Author(s): |
Ricardo Alves MESQUITA
[1]
;
Evelyn K. ANZAI
[2]
;
Rogério Nogueira OLIVEIRA
[3]
;
Fábio Daumas NUNES
[4]
Total Authors: 4
|
| Affiliation: | [1] Universidade de São Paulo. Faculdade de Odontologia
[2] Universidade de São Paulo. Faculdade de Odontologia
[3] Universidade de São Paulo. Faculdade de Odontologia
[4] Universidade de São Paulo. Faculdade de Odontologia
Total Affiliations: 4
|
| Document type: | Journal article |
| Source: | Pesquisa Odontológica Brasileira; v. 15, n. 4, p. 314-318, 2001-12-00. |
| Abstract | |
There are several protocols reported in the literature for the extraction of genomic DNA from formalin-fixed paraffin-embedded samples. Genomic DNA is utilized in molecular analyses, including PCR. This study compares three different methods for the extraction of genomic DNA from formalin-fixed paraffin-embedded (inflammatory fibrous hyperplasia) and non-formalin-fixed (normal oral mucosa) samples: phenol with enzymatic digestion, and silica with and without enzymatic digestion. The amplification of DNA by means of the PCR technique was carried out with primers for the exon 7 of human keratin type 14. Amplicons were analyzed by means of electrophoresis in an 8% polyacrylamide gel with 5% glycerol, followed by silver-staining visualization. The phenol/enzymatic digestion and the silica/enzymatic digestion methods provided amplicons from both tissue samples. The method described is a potential aid in the establishment of the histopathologic diagnosis and in retrospective studies with archival paraffin-embedded samples. (AU) | |