Protocol for DNA extraction of Cryptosporidium spp. oocysts in fecal samples

Author(s)

Elenice M.N. Gonçalves ^[1] ; Ronalda S. Araújo ^[2] ; Magali Orban ^[3] ; Glavur R. Matté ^[4] ; Maria Helena Matté ^[5] ; Carlos E.P. Corbett ^[6]

Affiliation

^[1] Universidade de São Paulo. Faculdade de Medicina. Hospital das Clínicas - Brasil

^[2] Universidade de São Paulo. Faculdade de Saúde Pública. Laboratório de Saúde Pública - Brasil

^[3] Universidade de São Paulo. Faculdade de Medicina. Hospital das Clínicas - Brasil

^[4] Universidade de São Paulo. Faculdade de Saúde Pública. Laboratório de Saúde Pública - Brasil

^[5] Universidade de São Paulo. Faculdade de Saúde Pública. Laboratório de Saúde Pública - Brasil

^[6] Universidade de São Paulo. Faculdade de Medicina. Laboratório de Patologia das Doenças Infecciosas - Brasil

• Document type: Journal article
• Source: Revista do Instituto de Medicina Tropical de São Paulo; v. 50, n. 3, p. 165-167, 2008-05-14.

Abstract

Molecular characterization of Cryptosporidium spp.oocysts in clinical samples is useful for public health since it allows the study of sources of contamination as well as the transmission in different geographical regions. Although widely used in developed countries, in Brazil it is restricted to academic studies, mostly using commercial kits for the extraction of genomic DNA, or in collaboration with external reference centers, rendering the method expensive and limited. The study proposes the application of the modifications recently introduced in the method improving feasibility with lower cost. This method was efficient for clinical samples preserved at -20 °C for up to six years and the low number of oocysts may be overcomed by repetitions of extraction. (AU)

• FAPESP's process: 05/03783-0 - Genotyping of Cryptosporidium spp. from environmental and clinical samples as source of data of species dispersion in the environment and clinic cases
• Grantee: Maria Helena Matte
• Support Opportunities: Regular Research Grants