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(Reference retrieved automatically from SciELO through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Performance of cervical cytology with review by different observers and hybrid capture II in the diagnosis of cervical intraepithelial neoplasia grades 2 and 3

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Author(s):
André Luís Ferreira Santos [1] ; Sophie Françoise Mauricette Derchain [2] ; Evelyn Bartholo Calvert [3] ; Marcos Roberto Martins [4] ; Rozany Mucha Dufloth [5] ; Edson Zangiacomi Martinez [6]
Total Authors: 6
Affiliation:
[1] Hospital Universitário de Taubaté - Brasil
[2] Universidade Estadual de Campinas. Faculdade de Ciências Médicas. Departamento de Tocoginecologia - Brasil
[3] Hospital Universitário de Taubaté - Brasil
[4] Hospital Universitário de Taubaté - Brasil
[5] Universidade Estadual de Campinas. Faculdade de Ciências Médicas. Departamento de Tocoginecologia - Brasil
[6] Universidade Estadual de Campinas. Faculdade de Ciências Médicas. Departamento de Tocoginecologia - Brasil
Total Affiliations: 6
Document type: Journal article
Source: Cadernos de Saúde Pública; v. 19, n. 4, p. 1029-1037, 2003-08-00.
Abstract

To evaluate the performance of initial cervical cytology and that collected at the reference service with a review conducted by different observers and techniques, as well as hybrid capture II, in the diagnosis of high-grade cervical intraepithelial neoplasia (CIN), 105 women attended from August 2000 to June 2001 for preneoplastic atypia upon cervical cytology were included. A new cervical cytology and hybrid capture II for DNA-HPV were conducted in all the patients. Cervical biopsies were taken in 91 women. Performance of the investigative procedures was described by estimating the sensitivity, specificity, and positive likelihood ratio (PLR), with a 95% confidence interval (95% CI), considering histological diagnosis the gold standard. Results: initial cervical cytology showed sensitivity and specificity of 57% and 82% for diagnosis of CIN grades 2 and 3, with a PLR of 3.2 (95% CI: 1.5-6.8). Referral cervical cytology showed a sensitivity and specificity for CIN 2 and 3 of 79% and 84%, respectively, with a PLR of 5.0 (95% CI: 2.5-10.0). Sensitivity (86%), specificity (80%), and PLR (4.3) were similar when a second observer (using a routine technique) reviewed the slide. Using rapid revision by a third observer, the sensitivity was significantly lower (64%). Hybrid capture II showed a high sensitivity (100%), low specificity (43%), and low PLR (1.7, 95% CI: 1.4-2.2). (AU)