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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Monitoring the Targeting of Cathepsin D to the Lysosome by Metabolic Labeling and Pulse-chase Analysis

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Author(s):
Tavares, Lucas A. [1] ; daSilva, Luis L. P. [1]
Total Authors: 2
Affiliation:
[1] Univ Sao Paulo, Ribeirao Preto Med Sch FMRP, Dept Cell & Mol Biol, Ribeirao Preto, SP - Brazil
Total Affiliations: 1
Document type: Journal article
Source: BIO-PROTOCOL; v. 7, n. 21 NOV 5 2017.
Web of Science Citations: 0
Abstract

Mannose 6-phosphate receptors function can be studied in living cells by investigating alterations in processing and secretion of their ligand Cathepsin D. The assay described here is well established in the literature and comprises the metabolic labeling of newly synthesized proteins with {[}S-35] methionine-cysteine in HeLa cells to monitor Cathepsin D processing through secretory pathway and secretion using immunoprecipitation, SDS-PAGE and fluorography. (AU)

FAPESP's process: 16/18207-9 - Characterization of protein trafficking pathways involved in lysosome biogenesis and function in human cells
Grantee:Lucas Alves Tavares
Support Opportunities: Scholarships in Brazil - Doctorate
FAPESP's process: 14/25812-0 - Study on the molecular interactions of HIV-1 with the endomembrane system of the host cells
Grantee:Luis Lamberti Pinto da Silva
Support Opportunities: Regular Research Grants