| Full text | |
| Author(s): |
Total Authors: 2
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| Affiliation: | [1] Univ Sao Paulo, Ribeirao Preto Med Sch FMRP, Dept Cell & Mol Biol, Ribeirao Preto, SP - Brazil
Total Affiliations: 1
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| Document type: | Journal article |
| Source: | BIO-PROTOCOL; v. 7, n. 21 NOV 5 2017. |
| Web of Science Citations: | 0 |
| Abstract | |
Mannose 6-phosphate receptors function can be studied in living cells by investigating alterations in processing and secretion of their ligand Cathepsin D. The assay described here is well established in the literature and comprises the metabolic labeling of newly synthesized proteins with {[}S-35] methionine-cysteine in HeLa cells to monitor Cathepsin D processing through secretory pathway and secretion using immunoprecipitation, SDS-PAGE and fluorography. (AU) | |
| FAPESP's process: | 16/18207-9 - Characterization of protein trafficking pathways involved in lysosome biogenesis and function in human cells |
| Grantee: | Lucas Alves Tavares |
| Support Opportunities: | Scholarships in Brazil - Doctorate |
| FAPESP's process: | 14/25812-0 - Study on the molecular interactions of HIV-1 with the endomembrane system of the host cells |
| Grantee: | Luis Lamberti Pinto da Silva |
| Support Opportunities: | Regular Research Grants |