The mechanisms involved in the increased adiposity induced by interruption of regular physical exercise practice

Author(s)

Sertie, Rogerio A. L. ^[1] ; Curi, Rui ^[1] ; Oliveira, Ariclecio C. ^[2] ; Andreotti, Sandra ^[1] ; Caminhotto, Rennan O. ^[1] ; de Lima, Thais Martins ^[3] ; Proenca, Andre R. G. ^[4] ; Reis, Gabriela B. ^[1] ; Lima, Fabio B. ^[1]

Affiliation

^[1] Univ Sao Paulo, Inst Biomed Sci, Dept Physiol & Biophys, 1524 Prof Lineu Prestes Ave, BR-05508900 Sao Paulo, SP - Brazil

^[2] Univ Estadual Ceara, Super Inst Biomed Sci, Fortaleza, Ceara - Brazil

^[3] Univ Sao Paulo, Fac Med, LIM 51, Clin Emergencies Lab, Sao Paulo - Brazil

^[4] Univ Estadual Campinas, Fac Appl Sci, Lab Biotechnol, Limeira - Brazil

• Document type: Journal article
• Source: Life Sciences; v. 222, p. 103-111, APR 1 2019.
• Web of Science Citations: 1

Abstract

Aims: We investigated the effects of physical detraining on lipogenesis/lipolysis and cellularity (apoptosis/adipogenesis) in rat subcutaneous (inguinal; SC) and visceral (retroperitoneal; RP) white adipose depots. Main methods: Three groups of male Wistar rats (6-wk old) were studied: (1) (T) trained for 12 weeks; (2) (D) trained for 8 weeks and detrained for 4 weeks; and (3) (S) age-matched sedentary. Training consisted of treadmill running sessions (1 h/day, 5 days/week, 50-60% maximal race capacity). Key findings: Physical detraining increased glucose oxidation, lipogenesis, and adipocyte size in the SC and RP depots. The number of apoptotic SC adipocytes was reduced by 53% in the T (p < 0.0001) and by 43% in the D (p < 0.001) as compared with S. RP adipocyte apoptosis in the T and D was 9.48% and 10.9% greater compared to the S, respectively (p < 0.05). In the SC stromal vascular fraction (SVF) of D rats, adiponectin, sterol regulatory element binding protein (SREBP)-1c, Peroxisome proliferator-activated receptor gamma (PPAR.), and Perilipin A mRNA expressions were more pronounced than S group, suggesting a more intense adipogenesis. This putative adipogenic effect was not observed in the RP depot. The physical detraining promoted rapid increase in the SC and RP depots however not through the same mechanisms. Significance: Physical detraining induced fat cell hypertrophy (increase of lipogenesis) in both SC and RP whereas hyperplasia (increase of adipogenesis and reduction of apoptosis) was found in SC only. These results indicate the mechanism associated with obesogenic effects of detraining varies with the fat depot. (AU)

• FAPESP's process: 13/13601-2 - The influence of chronic aerobic training and physical detraining on adiposity in subcutaneous and retroperitoneal rat fat pad: characterization of apoptosis and adipogenesis
• Grantee: Rogério Antônio Laurato Sertié
• Support Opportunities: Scholarships in Brazil - Post-Doctoral