Advanced search
Start date
Betweenand
Related content
(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

The Long Pentraxin 3 Contributes to Joint Inflammation in Gout by Facilitating the Phagocytosis of Monosodium Urate Crystals

Full text
Author(s):
Show less -
Batista, V, Nathalia ; Barbagallo, Marialuisa [1] ; Oliveira, Vivian L. S. [2] ; Castro-Gomes, Thiago [3] ; Oliveira, Rene D. R. [4] ; Louzada-Junior, Paulo [4] ; Pinheiro, Geraldo R. C. [5] ; Mantovani, Alberto [6, 7] ; Teixeira, Mauro M. [2] ; Garlanda, Cecilia [1, 6] ; Amaral, Flavio A. [2]
Total Authors: 11
Affiliation:
[1] Humanitas Res Hosp, I-20089 Rozzano - Italy
[2] Batista, Nathalia, V, Univ Fed Minas Gerais, Inst Biol Sci, Dept Biochem & Immunol, Lab Immunopharmacol, BR-31270901 Belo Horizonte, MG - Brazil
[3] Univ Fed Minas Gerais, Inst Biol Sci, Dept Parasitol, BR-31270901 Belo Horizonte, MG - Brazil
[4] Univ Sao Paulo, Ribeirao Preto Med Sch, Div Rheumatol, BR-1409900 Sao Paulo - Brazil
[5] Univ Estado Rio De Janeiro, Discipline Rheumatol, BR-20550900 Rio De Janeiro - Brazil
[6] Humanitas Univ, Via Levi Montalcini, I-20090 Pieve Emanuele - Italy
[7] Queen Mary Univ London, William Harvey Res Inst, London EC1M 6BQ - England
Total Affiliations: 7
Document type: Journal article
Source: JOURNAL OF IMMUNOLOGY; v. 202, n. 6, p. 1807-1814, MAR 15 2019.
Web of Science Citations: 0
Abstract

The purpose of this study was to investigate the role of pentraxin 3 (PTX3), a pivotal component of the innate immune system, in gout. Levels of PTX3 and IL-1 beta in human samples were evaluated by ELISA. Development of murine gout was evaluated through the levels of cytokines (PTX3, CXCL1, and IL-1 beta) and neutrophil recruitment into the joint cavity. Phagocytosis of monosodium urate (MSU) crystals and caspase-1 activation were determined by flow cytometer. Acute gout patients showed elevated concentration of PTX3 in plasma and synovial fluid as compared with healthy and osteoarthritic subjects. Moreover, there was a positive correlation between intra-articular PTX3 and IL-1 beta levels. PTX3 was induced in the periarticular tissue of mice postinjection of MSU crystals. Importantly, Ptx3-deficient mice showed reduced inflammation in response to MSU crystal injection compared with wild-type mice, including reduction of neutrophil recruitment into the joint cavity and IL-1 beta and CXCL1 production. Interestingly, addition of PTX3 in vitro enhanced MSU crystal phagocytosis by monocytes and resulted in higher production of IL-1 beta by macrophages. This contribution of PTX3 to the phagocytosis of MSU crystals and consequent production of IL-1 beta occurred through a mechanism mainly dependent on Fc gamma RIII. Thus, our results suggest that PTX3 acts as a humoral pattern recognition molecule in gout facilitating MSU crystal phagocytosis and contributing to the pathogenesis of gouty arthritis. (AU)

FAPESP's process: 13/08216-2 - CRID - Center for Research in Inflammatory Diseases
Grantee:Fernando de Queiroz Cunha
Support Opportunities: Research Grants - Research, Innovation and Dissemination Centers - RIDC