Biological response of chemically treated surface of the ultrafine-grained Ti-6Al-7Nb alloy for biomedical applications

Author(s)

de Oliveira, Diego Pedreira ^[1] ; Toniato, Tatiane Venturott ^[2] ; Ricci, Ritchelli ^[2] ; Marciano, Fernanda Roberta ^[3] ; Prokofiev, Egor ^[4] ; Valiev, Ruslan Z. ^{[5, 4]} ; Lobo, Anderson Oliveira ^[6] ; Jorge Junior, Alberto Moreira ^{[1, 7, 8]}

Affiliation

^[1] Univ Fed Sao Carlos, Dept Mat Engn, BR-13565905 Sao Carlos, SP - Brazil

^[2] Univ Vale do Paraiba, Inst Res & Dev, BR-12244000 Sao Paulo - Brazil

^[3] Brasil Univ, Sci & Technol Inst, BR-08230030 Sao Paulo - Brazil

^[4] St Petersburg State Univ, St Petersburg 199034 - Russia

^[5] Ufa State Aviat Tech Univ, Inst Phys Adv Mat, Ufa 450000 - Russia

^[6] UFPI Fed Univ Piaui, LIMAV Interdisciplinary Lab Adv Mat, Dept Mat Engn, BR-64049550 Teresina, Piaui - Brazil

^[7] Univ Grenoble Alpes, CNRS, Grenoble INP, LEPMI, F-38000 Grenoble - France

^[8] SIMAP Labs, F-38000 Grenoble - France

• Document type: Journal article
• Source: INTERNATIONAL JOURNAL OF NANOMEDICINE; v. 14, p. 1725-1736, 2019.
• Web of Science Citations: 0

Abstract

Background: Nanophase surface properties of titanium alloys must be obtained for a suitable biological performance, particularly to facilitate cell adhesion and bone tissue formation. Obtaining a bulk nanostructured material using severe plastic deformation is an ideal processing route to improve the mechanical performance of titanium alloys. By decreasing the grain size of a metallic material, a superior strength improvement can be obtained, while surface modification of a nanostructured surface can produce an attractive topography able to induce biological responses in osteoblastic cells. Methods: Aiming to achieve such an excellent synergetic performance, a processing route, which included equal channel angular pressing (ECAP), hot and cold extrusion, and heat treatments, was used to produce a nanometric and ultrafine-grained (UFG) microstructure in the Ti-6Al-7Nb alloy (around of 200 nm). Additionally, UFG samples were surface-modified with acid etching (UFG-A) to produce a uniform micron and submicron porosity on the surface. Subsequently, alkaline treatment (UFG-AA) produced a sponge-like nanotopographic substrate able to modulate cellular interactions. Results: After several kinds of biological tests for both treatment conditions (UFG-A and UFG-AA), the main results have shown that there was no cytotoxicity, expressed alkaline phosphatase activity and total protein amounts without statistical differences compared to control. However, the UFG-AA samples presented an attractive effect on the cell membranes, and cell adhesions were preferentially induced as compared with UFG-A. Both conditions demonstrated cell projections, but for UFG-AA, cells were more widely dispersed, and more quantities of filopodia formation could be observed. Conclusion: Herein, the reasons for such behaviors are discussed, and further results are presented in addition to those mentioned above. (AU)

• FAPESP's process: 13/06258-0 - Development of surfaces anodically oxidized of titanium alloys and modified femtosecond laser and fatigue resistant
• Grantee: Diego Pedreira de Oliveira
• Support Opportunities: Scholarships in Brazil - Post-Doctoral