| Full text | |
| Author(s): |
Alexandre, Pamela A.
[1, 2]
;
Naval-Sanchez, Marina
[2]
;
Porto-Neto, Laercio R.
[2]
;
Ferraz, Jose Bento S.
[1]
;
Reverter, Antonio
[2]
;
Fukumasu, Heidge
[1]
Total Authors: 6
|
| Affiliation: | [1] Univ Sao Paulo, Dept Vet Med, Coll Anim Sci & Food Engn, Pirassununga - Brazil
[2] CSIRO, Agr & Food, Brisbane, Qld - Australia
Total Affiliations: 2
|
| Document type: | Journal article |
| Source: | FRONTIERS IN GENETICS; v. 10, MAR 22 2019. |
| Web of Science Citations: | 1 |
| Abstract | |
Systems biology approaches are used as strategy to uncover tissue-specific perturbations and regulatory genes related to complex phenotypes. We applied this approach to study feed efficiency (FE) in beef cattle, an important trait both economically and environmentally. Poly-A selected RNA of five tissues (adrenal gland, hypothalamus, liver, skeletal muscle and pituitary) of eighteen young bulls, selected for high and low FE, were sequenced (Illumina HiSeq 2500, 100 bp, pared-end). From the 17,354 expressed genes considering all tissues, 1,335 were prioritized by five selection categories (differentially expressed, harboring SNPs associated with FE, tissue-specific, secreted in plasma and key regulators) and used for network construction. NR2F6 and TGFB1 were identified and validated by motif discovery as key regulators of hepatic inflammatory response and muscle tissue development, respectively, two biological processes demonstrated to be associated with FE. Moreover, we indicated potential biomarkers of FE, which are related to hormonal control of metabolism and sexual maturity. By using robust methodologies and validation strategies, we confirmed the main biological processes related to FE in Bos indicus and indicated candidate genes as regulators or biomarkers of superior animals. (AU) | |
| FAPESP's process: | 17/14707-0 - Multi-tisssue omics analyses to uncover feed efficiency in beef cattle |
| Grantee: | Pâmela Almeida Alexandre |
| Support Opportunities: | Scholarships abroad - Research Internship - Doctorate |
| FAPESP's process: | 15/22276-3 - Systems Biology Approach to Determine Molecular Mechanisms Associated with Feed Efficiency in Nelore Cattle |
| Grantee: | Pâmela Almeida Alexandre |
| Support Opportunities: | Scholarships in Brazil - Doctorate |
| FAPESP's process: | 14/07566-2 - Genomics applied to ruminant production |
| Grantee: | José Bento Sterman Ferraz |
| Support Opportunities: | Research Projects - Thematic Grants |
| FAPESP's process: | 14/02493-7 - Mammary tumors of dogs and the cancer stem cell theory: a comparative and translational approach |
| Grantee: | Heidge Fukumasu |
| Support Opportunities: | Research Grants - Young Investigators Grants |