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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Diversity of Anaplasma species in cattle in Mozambique

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Author(s):
Fernandes, Simone de Jesus [1] ; Matos, Carlos Antonio [1, 2] ; Freschi, Carla Roberta [3] ; de Souza Ramos, Inalda Angelica [1] ; Machado, Rosangela Zacarias [1] ; Andre, Marcos Rogerio [1]
Total Authors: 6
Affiliation:
[1] Paulista State Univ Unesp, Fac Agrarian & Vet Sci, Univ Estadual Paulista Unesp, Jaboticabal, SP - Brazil
[2] Directorate Anim Sci, Maputo - Mozambique
[3] Imunodot Diagnost Ltda, Jaboticabal, SP - Brazil
Total Affiliations: 3
Document type: Journal article
Source: TICKS AND TICK-BORNE DISEASES; v. 10, n. 3, p. 651-664, APR 2019.
Web of Science Citations: 1
Abstract

Although species of Anaplasma are highly prevalent Rickettsiales agents in domestic and wild ruminants with a wide distribution worldwide, few studies have been conducted so far to detect and/or investigate the diversity of these agentsin cattle in Mozambique. In the present study, serological and molecular assays were used to investigate the occurrence of Anaplasma spp. in 219 bovines sampled in the districts of Boane, Magude, Matutuine, Moamba and Namaacha in Maputo, Mozambique. In the iELISA test for detection ofIgG antibodies to A. marginale, 86.3% (189/219) of the samples were positive. In qPCR assays for the gene msp1 beta for A. marginale and msp2 for A. phagocytophilum, 97.3% (213/219) and 2.7% (6/219) of the animals were positive, respectively. Two different cPCR protocols based on the 16S rRNA gene showed that 100% of the samples were positive for Anaplasma spp. The DNA sequences obtained were phylogenetically related to A. platys, A. phagocytophilum, Candidatus Anaplasma boleense, A. centrale, A. marginale and A. ovis. Phylogenetic inference based on the msp4 and msp5 genes positioned the obtained sequences in the clade of A. marginale, with evidence of occurrence of 8 and 5 different haplotypes for each gene, respectively. Anaplasma sp. phylogenetically associated with A. platys was evidenced in phylogenetic analyzes based on 16S rRNA and groEL genes. It is concluded that a high diversity of species of Anaplasma spp. occurs in cattle in Mozambique. (AU)

FAPESP's process: 15/14896-1 - Molecular detection and characterization of agents belonging to Anaplasmataceae e Bartonellaceae family in wild animals and ectoparasites in Brazil
Grantee:Marcos Rogério André
Support Opportunities: Regular Research Grants