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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Protein and mRNA detection of classic cytokines in corresponding samples of serum, gingival tissue and gingival crevicular fluid from subjects with periodontitis

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Author(s):
Duarte, Poliana Mendes [1] ; Abreu, Leticia de Lorenzo [1] ; Vilela, Andre [1] ; Feres, Magda [1] ; Giro, Gabriela [1] ; Miranda, Tamires Szeremeske [1]
Total Authors: 6
Affiliation:
[1] Univ Guarulhos, Dent Res Div, Dept Periodontol, Guarulhos - Brazil
Total Affiliations: 1
Document type: Journal article
Source: JOURNAL OF PERIODONTAL RESEARCH; v. 54, n. 2, p. 174-179, APR 2019.
Web of Science Citations: 1
Abstract

Background and objectiveLittle is known about the correspondence of cytokines detected among the different biological matrices used in periodontal research and whether locally-detected cytokine mRNA can also be identified at the translated protein level. The aims of this study were to compare: (a) the detection of classic cytokines at protein level in corresponding samples of serum, gingival tissue and gingival crevicular fluid (GCF) in subjects with periodontitis and (b) the detection of cytokines at protein level in GCF and tissue with their detection at mRNA level in tissue. Material and methodsProtein concentrations of IL-1, IL-6 and TNF- were measured in corresponding samples of GCF, gingival tissue and serum from 125 subjects with chronic periodontitis by multiplex immunoassay. The mRNA levels of these cytokines were evaluated in the same gingival tissue by qPCR. ResultsThe mean protein concentrations of IL-1 ss and TNF- were higher in tissue and GCF than in serum (P<0.05). The mean protein concentration of IL-6 was higher in tissue, followed by serum and GCF, respectively (P<0.05). Using serum as the gold standard, detection of IL-1 ss in GCF and IL-6 in tissue had the highest sensitivity (100%), while detection of IL-6 in the GCF had the lowest sensitivity (77.9%). Using tissue as the gold standard, the detection sensitivities of IL-1 ss, IL-6 and TNF- were 93.6%, 78.4% and 94.2%, respectively, in GCF. Using mRNA as the gold standard, the detection of IL-1 ss and IL-6 at the protein level in tissue (100.0%) had the highest sensitivity, while the detection of IL-6 in GCF (77.9%) had the lowest sensitivity. ConclusionTissue and GCF exhibited overall higher protein concentrations of cytokines than serum. GCF presented good reliability for identifying the target cytokines at protein level, when compared with detecting tissue cytokines at protein level. GCF and tissue presented high sensitivities for detecting cytokines at the protein level, when compared with detecting tissue cytokines at the mRNA level. The sensitivity for the identification of cytokines in the GCF depended on the target cytokine, where IL-6 was the least likely cytokine to be detected in this matrix. (AU)

FAPESP's process: 17/00067-9 - Correlation between the levels of pro-inflammatory cytokines in serum, tissue and gingival crevicular fluid in patients with chronic periodontitis
Grantee:Letícia de Lorenzo Abreu
Support Opportunities: Scholarships in Brazil - Scientific Initiation