Advanced search
Start date
Betweenand

Analysis of cytokines (in gingival fluid and serum) and periodontal pathogens (Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, Tannerella forsythia, and Treponema denticola) in patients with aggressive periodontitis. longitudinal study

Grant number: 13/26381-0
Support type:Regular Research Grants
Duration: November 01, 2014 - October 31, 2016
Field of knowledge:Health Sciences - Dentistry - Periodontology
Principal researcher:Luciana Saraiva
Grantee:Luciana Saraiva
Home Institution: Faculdade de Odontologia (FO). Universidade de São Paulo (USP). São Paulo , SP, Brazil
Assoc. researchers: Eder de Souza Martins ; Marcia Pinto Alves Mayer ; Marinella Holzhausen Caldeira ; Ricardo Takiy Sekiguchi

Abstract

Many researches on periodontal disease have sought a correlation between local and systemic factors. In Aggressive Periodontitis (AP), an exacerbated inflammatory response to some microorganisms has been reported, however, still little is known about the mechanisms linking these factors. The objectives of this study will be to evaluate some cytokines in gingival crevicular fluid (GCF) and serum of patients with AP (localized and generalized) and healthy subjects. In addition to the clinical data, samples will be obtained from peripheral blood and GCF of two disease sites for analysis of the cytokines and from subgingival biofilm to detect and quantify, through the Real-Time PCR, four periodontal pathogens: Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, Tannerella forsythia, and Treponema denticola. Furthermore it will be do a research for A. actinomycetemcomitans clone JP2 due to its strong association with AP. Kits for detection of human cytokines (Human Luminex Screening Assay - R&D Systems Inc., Minneapolis, USA)) will be used for detection of cytokines in GCF and serum in order to verify the existence of an association between local and sera mediators in healthy subjects and patients with AP. The analyzed cytokines will be: IL-1², IL-8, IL-10, IL-11, IL-17, ICAM-1, MCP-1, M-CSF and GM-CSF, among others. The hypothesis is that there is a correlation between cytokines related to AP found in GCF and serum and that the serum could serve as predictors of disease. (AU)

Scientific publications (4)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
MARTINS, E. S.; CESAR-NETO, J. B.; ALBUQUERQUE-SOUZA, E.; REBEIS, E. S.; HOLZHAUSEN, M.; PANNUTI, C. M.; MAYER, M. P. A.; SARAIVA, L. One-year follow-up of the immune profile in serum and selected sites of generalized and localized aggressive periodontitis. CYTOKINE, v. 116, p. 27-37, APR 2019. Web of Science Citations: 1.
REBEIS, ESTELA SANCHES; ALBUQUERQUE-SOUZA, EMMANUEL; DA SILVA, MAIKE PAULINO; GIUDICISSI, MARCELA; MAYER, MARCIA P. A.; SARAIVA, LUCIANA. Effect of periodontal treatment on Aggregatibacter actinomycetemcomitans colonization and serum IgG levels against A-actinomycetemcomitans serotypes and Omp29 of aggressive periodontitis patients. ORAL DISEASES, v. 25, n. 2, p. 569-579, MAR 2019. Web of Science Citations: 1.
LUCAS MACEDO BATITUCCI AMBRÓSIO; EMANUEL DA SILVA ROVAI; BRUNO NUNES DE FRANÇA; DANILO ANDRÉS BALZARINI; IEDA SANTOS ABREU; SHEYLA BATISTA BOLOGNA LOPES; THAÍS BORGUEZAN NUNES; SILVIA VANESSA LOURENÇO; SANDRA GOFINET PASOTO; LUCIANA SARAIVA; MARINELLA HOLZHAUSEN. Effects of periodontal treatment on primary sjȫgren’s syndrome symptoms. Brazilian Oral Research, v. 31, p. -, 2017.
BATITUCCI AMBROSIO, LUCAS MACEDO; ROVAI, EMANUEL DA SILVA; DE FRANCA, BRUNO NUNES; BALZARINI, DANILO ANDRES; ABREU, IEDA SANTOS; BOLOGNA LOPES, SHEYLA BATISTA; NUNES, THAIS BORGUEZAN; LOURENCO, SILVIA VANESSA; PASOTO, SANDRA GOFINET; SARAIVA, LUCIANA; HOLZHAUSEN, MARINELLA. Effects of periodontal treatment on primary sjogren's syndrome symptoms. Brazilian Oral Research, v. 31, 2017. Web of Science Citations: 3.

Please report errors in scientific publications list by writing to: cdi@fapesp.br.