The aim of this study is to evaluate the effect of red complex bacterial species of periodontal microbiota and Aggregatibacter actinomycetemcomitans on the membrane phenotype and function of dendritic cells (DCs) maturated in vitro from peripheral blood mononuclear cells (PBMC) of the patients with aggressive periodontitis. Cultured human monocyte-derived dendritic cells (MDDCs) from periodontally-healthy and aggressive periodontitis subjects will be pulsed with extract of Tannarella forsythia, Porphyromonas gingivalis, Treponema denticola e Aggregatibacter actinomycetemcomitans. The effect of this treatment under the immunostimulate capacity of DCs will be evaluated in co-cultures with allogeneic T cells. The following cytokines will be quantified on the supernatant through ELISA test: IFN-γ, IL-10, TNF, IL-1 and IL-12. To determinate the cells’ phenotype, they will be marked with monoclonais antibodies: anti-CD1a, anti-CD14, anti-CD80, anti-CD86, anti-CD83, anti-CD11c, anti-CCR7 and class II- anti-HLA.
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