Microbial diversity of supra and subgingival biofilms and protein profile of saliva and acquired pellicle of subjects with aggressive periodontitis

• Grant number: 15/00259-0
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: June 01, 2015
• End date: July 31, 2019
• Field of knowledge: Biological Sciences - Microbiology - Applied Microbiology
• Agreement: Coordination of Improvement of Higher Education Personnel (CAPES)
• Principal Investigator: Marcia Pinto Alves Mayer
• Grantee: Pâmela Pontes Penas Amado
• Host Institution: Instituto de Ciências Biomédicas (ICB). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Associated scholarship(s): 17/26073-5 - Proteome of saliva and acquired pellicle and its correlation with the microbiome of supra and subgingival biofilms in aggressive periodontitis, BE.EP.DR

Abstract

Despite the association of aggressive periodontitis with known periodontal pathogens, particularly Aggregatibacter actinomycetemcomitans, and the involvement of host susceptibility with the periodontal, other differences in the composition of the oral microbiota may be involved with AP. Patients with aggressive periodontitis harbor low numbers of bacteria considered beneficial, particularly of the genera Streptococcus and Actinomyces, found in abundance in the oral biofilm of patients with normal periodontium. The initial adhesion of bacteria to dental surfaces is dependent of the composition of the enamel acquired pellicle, formed by proteins present in whole saliva. The present study aims to test the hypothesis that differences in the acquired pellicle composition between patients with aggressive periodontitis and normal periodontium are associated to differences in the composition of oral biofilm. Materials and Methods: The diversity of the supra and subgingival microbiota of patients with aggressive periodontitis will be analysed by 16srRNA sequencing and will be correlated with the profile of salivary and enamel acquired pellicle proteins. Five patients with localized aggressive periodontitis (PAL), less than 21 years old, carrying high levels of A. actinomycetemcomitans and low levels of other studied organisms will be selected. As control, 5 subjects with normal periodontium with no detectable levels of A.actinomycetemcomitans will be selected, matching to the aggressive periodontitis group in age, gender and race. Biofilm samples of supra and subgingival sites, parotid saliva, whole saliva and acquired pellicle will be collected. Analysis of supra and subgingival biofilm diversity of healthy and periodontitis sites will be performed after amplification of 16S rRNA followed by high throughput sequencing. The proteins profiles of parotid saliva, whole saliva and acquired pellicle will be determined by mass spectrometry and compared to the microbial composition of the supra and subgingival biofilm. Innovation: Differences in the composition of saliva among individuals can enable the discovery of proteins that promote colonization by beneficial bacteria instead of pathogens. These could be used in oral rinses in order to change the composition of dental biofilm, particularly in those families/populations with higher prevalence of aggressive periodontitis. In addition, beneficial bacteria associated with health can be identified to be used as probiotic agents against periodontal pathogens. Justification: Despite the low prevalence of aggressive periodontitis, this is a disease with family aggregation that leads to early tooth loss. So far, there are no effective preventive methods for patients from families with aggressive periodontitis. The mechanical treatment and antibiotic therapy are not completely effective, and should be performed throughout the life of the individual. Periodontitis is associated not only tooth loss, but is associated with various systemic conditions. This approach may in future be used in patients with chronic periodontitis, as stated in the grant proposal, simultaneously to this scholarship request. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

AMADO, PAMELA PONTES PENAS; KAWAMOTO, DIONE; ALBUQUERQUE-SOUZA, EMMANUEL; FRANCO, DIEGO CASTILLO; SARAIVA, LUCIANA; CASARIN, RENATO CORREA VIANA; HORLIANA, ANNA CAROLINA RATTO TEMPESTINI; MAYER, MARCIA PINTO ALVES. Oral and Fecal Microbiome in Molar-Incisor Pattern Periodontitis. FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY, v. 10, OCT 8 2020. (15/00259-0, 16/14687-6, 16/13159-6, 15/18273-9)

KAWAMOTO, DIONE; LUCAS AMADO, PAMELA PONTES; ALBUQUERQUE-SOUZA, EMMANUEL; BUENO, MANUELA ROCHA; VALE, GLAUBER CAMPOS; SARAIVA, LUCIANA; ALVES MAYER, MARCIA PINTO. Chemokines and cytokines profile in whole saliva of patients with periodontitis. CYTOKINE, v. 135, p. 9-pg., 2020-11-01. (16/13159-6, 17/16377-7, 15/00259-0, 17/10829-3, 15/18273-9)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

AMADO, Pâmela Pontes Penas. Diversity analysis of the supra and subgingival microbiota and proteins profile of saliva and acquired enamel pellicle of individuals with periodontitis.. 2019. Doctoral Thesis - Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI) São Paulo.