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Proteome of saliva and acquired pellicle and its correlation with the microbiome of supra and subgingival biofilms in aggressive periodontitis

Grant number: 17/26073-5
Support type:Scholarships abroad - Research Internship - Doctorate
Effective date (Start): March 12, 2018
Effective date (End): February 28, 2019
Field of knowledge:Biological Sciences - Microbiology - Applied Microbiology
Principal Investigator:Marcia Pinto Alves Mayer
Grantee:Pâmela Pontes Penas Amado
Supervisor abroad: Walter Luiz Siqueira
Home Institution: Instituto de Ciências Biomédicas (ICB). Universidade de São Paulo (USP). São Paulo , SP, Brazil
Local de pesquisa : Western University , Canada  
Associated to the scholarship:15/00259-0 - Microbial diversity of supra and subgingival biofilms and protein profile of saliva and acquired pellicle of subjects with aggressive periodontitis, BP.DR

Abstract

Initial adhesion of bacteria to the dental surface is dependent on the acquired enamel pellicle, formed by proteins present in whole saliva. Studies on the microbiology of aggressive periodontitis indicate an increased proportion of periodontopathogens such as A.actinomycetemcomitans and P. gingivalis, but also decreased proportions of early colonizers such as streptococci and other beneficial organisms such as Actinomyces. The present study aims to test the hypothesis that the oral microbiota in aggressive periodontitis is modulated by differences in the acquired pellicle and saliva. The proteome of whole saliva (stimulated and unstimulated), parotid saliva and acquired pellicle will be evaluated by mass spectrometry. Samples were obtained from of 7 patients with localized aggressive periodontitis and 7 control subjects with healthy periodontium, paired by age, gender and race. Samples will be prepared and in-solution digestion will be performed. Peptide separation and mass spectrometric analyses will be carried out by LC-ESI-MS/MS (Liquid Chromatography Electrospray Ionization Tandem Mass Spectrometry). The obtained MS/MS spectra will be searched against human protein databases (Swiss Prot and TrEMBL, Swiss Institute of Bioinformatics, Geneva, Switzerland, http://ca.expasy.org/sprot/). (AU)