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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Memory of Periodic Thermal Stimulation in an Immune Complex

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Author(s):
Stan, Razvan C. [1, 2] ; de Camargo, Maristela M. [1]
Total Authors: 2
Affiliation:
[1] Univ Sao Paulo, Immunol Dept, Sao Paulo - Brazil
[2] Cantacuzino Mil Med Res Dev Natl Inst, Bucharest - Romania
Total Affiliations: 2
Document type: Journal article
Source: CHEMISTRYSELECT; v. 4, n. 12, p. 3325-3328, MAR 29 2019.
Web of Science Citations: 1
Abstract

Proteins search their conformational space in order to attain the native fold and bind productively to relevant biological partners. Most proteins must be able to alternate between at least one active conformational state back to an inactive conformer, especially for macromolecules that perform work and need to optimize energy usage. This property may be invoked by a physical stimulus (eg. temperature) or by a chemical ligand, and may occur through mapping of the protein external environment onto a subset of protein conformers. We have stimulated with temperature cycles two partners of an immune complex before and after assembly. We have revealed with calorimetry that properties of the external stimulus are also found in the characteristics of the immune complex (i. e. periodic variations in the binding affinity). These results are important for delineating the bases of molecular memory ex vivo and may serve in the optimization of protein based sensors. (AU)

FAPESP's process: 11/51778-6 - Functional study of the Unfolded Protein Response in human b lymphocytes: primary hypogammaglobulinemia as a model for dysfunctional UPR
Grantee:Maristela Martins de Camargo
Support Opportunities: Regular Research Grants