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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Use of mucus as a non-invasive sampling method for DNA barcoding of stingrays and skates (batoid elasmobranchs)

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Author(s):
Domingues, Rodrigo R. [1] ; Garrone-Neto, Domingos [2, 3] ; Hilsdorf, Alexandre W. S. [4] ; Gadig, Otto B. F. [3]
Total Authors: 4
Affiliation:
[1] UNIFESP Univ Fed Sao Paulo, Inst Mar, Dept Ciencias Mar, Sao Paulo - Brazil
[2] UNESP Univ Estadual Paulista, Campus Expt Registro, Sao Paulo - Brazil
[3] UNESP Univ Estadual Paulista, Inst Biociencias, Lab Pesquisa Elasmobranquios, Campus Litoral Paulista, Sao Paulo - Brazil
[4] UMC, Nucleo Integrado Biotecnol, Sao Paulo - Brazil
Total Affiliations: 4
Document type: Journal article
Source: Journal of Fish Biology; v. 94, n. 3, p. 512-516, MAR 2019.
Web of Science Citations: 0
Abstract

In this study we tested the use of mucus from five species of Neotropical marine batoid elasmobranchs to extract genomic DNA for barcoding and phylogenetic analysis. The DNA from all individuals sampled was successfully amplified and sequenced for molecular barcode, allowing 99-100% accuracy to the species level. This method proved to provide reliable and good-quality DNA for barcoding and phylogenetic analysis of Neotropical elasmobranchs, through rapid handling and with low disturbance to animals. (AU)

FAPESP's process: 11/18513-9 - Use of unmanned submersible and acoustic telemetry for the study of elasmobranchs behavior in São Paulo, Brazil
Grantee:Otto Bismarck Fazzano Gadig
Support Opportunities: Regular Research Grants
FAPESP's process: 17/02420-8 - High-tech conservation: combining oceanography, transcriptome and genomics to assess population genetic connectivity and local adaptation of the highly migratory Mako shark (Isurus oxyrinchus) in the Atlantic Ocean
Grantee:Rodrigo Rodrigues Domingues
Support Opportunities: Scholarships in Brazil - Post-Doctoral
FAPESP's process: 13/08675-7 - Genetic Connectivity, Phylogeography and Pelagic Sharks Conservation in the Western Atlantic Ocean
Grantee:Rodrigo Rodrigues Domingues
Support Opportunities: Scholarships in Brazil - Doctorate