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(Reference retrieved automatically from SciELO through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Lack of association among TNF-α gene expression, -308 polymorphism (G > A) and virulence markers of Helicobacter pylori

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Author(s):
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Luanna Munhoz Zabaglia [1] ; Mariane Avante Ferraz [2] ; Weendelly Nayara Pereira [3] ; Wilson Aparecido Orcini [4] ; Roger Willian de Labio [5] ; Agostinho Caleman Neto [6] ; Fernanda Wisnieski [7] ; Juliana Garcia de Oliveira [8] ; Marilia de Arruda Cardoso Smith [9] ; Spencer Luiz Marques Payão ; Lucas Trevizani Rasmussen [11]
Total Authors: 11
Affiliation:
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[1] Universidade Sagrado Coração - Brasil
[2] Universidade Sagrado Coração - Brasil
[3] Universidade Sagrado Coração - Brasil
[4] Universidade Sagrado Coração - Brasil
[5] Faculdade de Medicina de Marília - Brasil
[6] Faculdade de Medicina de Marília - Brasil
[7] Universidade Federal de São Paulo - Brasil
[8] Universidade Sagrado Coração - Brasil
[9] Universidade Federal de São Paulo - Brasil
[11] Universidade Sagrado Coração - Brasil
Total Affiliations: 11
Document type: Journal article
Source: Journal of Venomous Animals and Toxins including Tropical Diseases; v. 21, 2015-12-31.
Abstract

Abstract Background Tumor necrosis factor plays a critical role in the pathogenesis of gastric diseases such as gastric cancer, and an abnormal inflammatory response has frequently been observed in dyspeptic patients. Helicobacter pylori infection can induce a gastric mucosal inflammatory response that may be influenced by -308 (G > A) polymorphisms and gene expression of theTNF-α gene. Methods One hundred and thirty-four gastric biopsy samples were collected from patients of both genders (61♂ and 73♀, mean age 40.3 ± 24.2 years) with gastric symptoms. The -308 (G > A) polymorphism of TNF-α was characterized using polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP). The expression level was measured using real-time PCR, and relative quantification (RQ) was calculated using the comparative CT method (2-ΔΔCT). Results The analysis revealed an increase in TNF-α gene expression in patients with gastritis; on the other hand, no statistical differences were observed in patients with gastric cancer. In addition, no association was found among -308 polymorphism genotypes, virulence markers, or TNF-α gene expression. Conclusions Helicobacter pylori induces a large increase in TNF-α expression in patients with gastritis, regardless of tissue inflammation, but after the tissue becomes neoplastic, the presence of bacteria did not influence expression. These results suggest that the TNF-α pathway may play an important role in the progression from gastritis to gastric cancer (AU)

FAPESP's process: 12/18333-3 - Characterization of virulence factor dupA, of Helicobacter pylori, genotyping and analysis of the expression of genes of tumor necrosis factor and E-cadherin in samples of children and adults with symptoms peptides
Grantee:Lucas Trevizani Rasmussen
Support Opportunities: Regular Research Grants
FAPESP's process: 13/21285-3 - Characterization of dupA gene of Helicobacter pylori and gene expression analysis of E-Caderin gene in gastric biopses samples from dyspeptic patients and gastric Cancer
Grantee:Mariane Avante Ferraz
Support Opportunities: Scholarships in Brazil - Scientific Initiation
FAPESP's process: 13/21224-4 - Genotyping and gene expression analysis of TNF-aslpha in samples from patients with gastric Disease: a possible relationship with Helicobacter pylori
Grantee:Luanna Munhoz Zabaglia
Support Opportunities: Scholarships in Brazil - Scientific Initiation