| Full text | |
| Author(s): Show less - |
Luanna Munhoz Zabaglia
[1]
;
Mariane Avante Ferraz
[2]
;
Weendelly Nayara Pereira
[3]
;
Wilson Aparecido Orcini
[4]
;
Roger Willian de Labio
[5]
;
Agostinho Caleman Neto
[6]
;
Fernanda Wisnieski
[7]
;
Juliana Garcia de Oliveira
[8]
;
Marilia de Arruda Cardoso Smith
[9]
;
Spencer Luiz Marques Payão
;
Lucas Trevizani Rasmussen
[11]
Total Authors: 11
|
| Affiliation: Show less - | [1] Universidade Sagrado Coração - Brasil
[2] Universidade Sagrado Coração - Brasil
[3] Universidade Sagrado Coração - Brasil
[4] Universidade Sagrado Coração - Brasil
[5] Faculdade de Medicina de Marília - Brasil
[6] Faculdade de Medicina de Marília - Brasil
[7] Universidade Federal de São Paulo - Brasil
[8] Universidade Sagrado Coração - Brasil
[9] Universidade Federal de São Paulo - Brasil
[11] Universidade Sagrado Coração - Brasil
Total Affiliations: 11
|
| Document type: | Journal article |
| Source: | Journal of Venomous Animals and Toxins including Tropical Diseases; v. 21, 2015-12-31. |
| Abstract | |
Abstract Background Tumor necrosis factor plays a critical role in the pathogenesis of gastric diseases such as gastric cancer, and an abnormal inflammatory response has frequently been observed in dyspeptic patients. Helicobacter pylori infection can induce a gastric mucosal inflammatory response that may be influenced by -308 (G > A) polymorphisms and gene expression of theTNF-α gene. Methods One hundred and thirty-four gastric biopsy samples were collected from patients of both genders (61♂ and 73♀, mean age 40.3 ± 24.2 years) with gastric symptoms. The -308 (G > A) polymorphism of TNF-α was characterized using polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP). The expression level was measured using real-time PCR, and relative quantification (RQ) was calculated using the comparative CT method (2-ΔΔCT). Results The analysis revealed an increase in TNF-α gene expression in patients with gastritis; on the other hand, no statistical differences were observed in patients with gastric cancer. In addition, no association was found among -308 polymorphism genotypes, virulence markers, or TNF-α gene expression. Conclusions Helicobacter pylori induces a large increase in TNF-α expression in patients with gastritis, regardless of tissue inflammation, but after the tissue becomes neoplastic, the presence of bacteria did not influence expression. These results suggest that the TNF-α pathway may play an important role in the progression from gastritis to gastric cancer (AU) | |
| FAPESP's process: | 12/18333-3 - Characterization of virulence factor dupA, of Helicobacter pylori, genotyping and analysis of the expression of genes of tumor necrosis factor and E-cadherin in samples of children and adults with symptoms peptides |
| Grantee: | Lucas Trevizani Rasmussen |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 13/21285-3 - Characterization of dupA gene of Helicobacter pylori and gene expression analysis of E-Caderin gene in gastric biopses samples from dyspeptic patients and gastric Cancer |
| Grantee: | Mariane Avante Ferraz |
| Support Opportunities: | Scholarships in Brazil - Scientific Initiation |
| FAPESP's process: | 13/21224-4 - Genotyping and gene expression analysis of TNF-aslpha in samples from patients with gastric Disease: a possible relationship with Helicobacter pylori |
| Grantee: | Luanna Munhoz Zabaglia |
| Support Opportunities: | Scholarships in Brazil - Scientific Initiation |