| Full text | |
| Author(s): |
Luciana Lamarão Damous
[1]
;
Sônia Maria da Silva
[2]
;
Renato Antônio Migliano Lopes
[3]
;
Célia Regina de Souza Bezerra Sakano
[4]
;
Manuel de Jesus Simões
[5]
;
Edna Frasson de Souza Montero
[6]
Total Authors: 6
|
| Affiliation: | [1] UNIFESP. Surgery and Research Postgraduate Program - Brasil
[2] UNIFESP. Surgery and Research Postgraduate Program - Brasil
[3] UNIFESP. Department of Pathology - Brasil
[4] UNIFESP. Gynecological Division - Brasil
[5] UNIFESP. Department of Morphology. Histology and Structural Biology Division - Brasil
[6] UNIFESP. Department of Surgery. Operative Technique and Experimental Surgery Division - Brasil
Total Affiliations: 6
|
| Document type: | Journal article |
| Source: | Acta Cirurgica Brasileira; v. 24, n. 5, p. 387-392, 2009-10-00. |
| Abstract | |
PURPOSE: To evaluate the remote ischemic preconditioning (R-IPC) impact on the quality of the ovarian graft by means of vaginal smear of transplanted rats. METHODS: Sixty rats were used divided in six groups: Control; Fresh transplant (TxF); Cryopreserved transplant (TxC); R-IPC; R-IPC + fresh transplant (TxF+R-IPC); R-IPC + cryopreserved transplant (TxC+R-IPC). R-IPC was performed in the common iliac artery. Autologous ovarian tissue was implanted integrally in the retro peritoneum. On the first PO day, vaginal smear collection was daily initiated. After 30 days, the PO day when the estrous cycle was re-initiated was considered for analysis as well as the estrous days and the number of estrous cycles. RESULTS: R-IPC showed a tendency to an early estrus re-initiation (p>0.05) as well as increase the number of cycles in the fresh transplanted group while in the cryopreserved transplant the number of cycles was similar, regardless of the stimulus R-IPC (p>0.05). The animals which had undergone fresh grafts had a longer estrous period than the ones which had undergone cryopreserved grafts, with or without R-IPC (p<0.05). CONCLUSION: R-IPC promoted earlier re-initiation of ovarian activity in the PO and greater estrous frequency, with more consistent results in the fresh grafts than in the cryopreserved ones. (AU) | |
| FAPESP's process: | 07/00107-9 - Ischemic preconditioning effect in autologous transplantation of rat ovaries |
| Grantee: | Edna Frasson de Souza Montero |
| Support Opportunities: | Regular Research Grants |