| Full text | |
| Author(s): |
Ana Carolina Firmino
[1]
;
Ivan Herman Fischer
[2]
;
Gabriel Leonardi Antonio
[3]
;
Quelmo Silva De Novaes
[4]
;
Hugo José Tozze Júnior
[5]
;
Edson Luis Furtado
[6]
Total Authors: 6
|
| Affiliation: | [1] Universidade Estadual Paulista "Júlio de Mesquita Filho". Faculdade de Ciências Agrárias e Tecnológicas - Brasil
[2] Agência Paulista de Tecnologia dos Agronegócios - Brasil
[3] Universidade Estadual Paulista "Júlio de Mesquita Filho". Faculdade de Ciências Agrárias e Tecnológicas - Brasil
[4] Universidade Estadual do Sudoeste da Bahia. Departamento de Fitotecnia e Zootecnia - Brasil
[5] Universidade Estadual Paulista "Júlio de Mesquita Filho". Faculdade de Ciências Agrárias e Tecnológicas - Brasil
[6] UNESP. Faculdade de Ciências Agronômicas. Departamento de Proteção Plantas - Brasil
Total Affiliations: 6
|
| Document type: | Journal article |
| Source: | Arq. Inst. Biol.; v. 83, 2016-12-15. |
| Abstract | |
ABSTRACT: Passion fruits (Passiflora edulis ) were found with symptoms of rot in the field, in the city of Tanhaçu, Bahia. After isolating the pathogen associated with this rot, in the present study we aimed to characterize the Ceratocystis isolate from passion fruit for better understanding this pathosystem. Molecular characterization was done based on the region ITS-5.8S rDNA. Pathogenic characterization was carried out for seedlings and fruits of passionflower. Passion fruit colonization was monitored by means of scanning electron microscopy techniques (SEM). DNA analysis of the Ceratocystis isolate from passionflower pointed out that this species belongs to Ceratocystis fimbriata . The inoculated passionflower seedlings showed injury at 30 days post-inoculation, but no inoculated plant showed wilt or died. Considering fruits, no differences were found for lesions caused by this fungus among cultivars, and lesions had average diameters of 1.0 and 2.2 cm at 7 and 11 days, respectively. The analysis using SEM indicated fungus spore germination and penetration in the fruit between 2 and 6 hours post-inoculation. At 12 and 24 hours post-inoculation, fruit colonization was noted both externally and internally, while fruit wall degradation started at 48 hours post-inoculation. At 90 hours post-inoculation, formation of new perithecia was observed inside and outside the fruit. This study complements the available information about the interaction of this fungus with passion fruit. (AU) | |
| FAPESP's process: | 11/05710-0 - Eucalyptus-Ceratocystis interaction: evaluation of clonal resistance, characterization of the Ceratocystis isolates and vector insect in Brazil |
| Grantee: | Ana Carolina Firmino |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |