Correlation between molecular features and genetic subtypes of Glioblastoma: critical analysis in 109 cases

Author(s)

Thais F Galatro ^[1] ; Paula Sola ^[2] ; Isabele F Moretti ^[3] ; Flavio K Miura ^[4] ; Sueli M Oba-Shinjo ^[5] ; Suely KN Marie ; Antonio M Lerario

Affiliation

^[1] Universidade de Sao Paulo. Faculdade de Medicina FMUSP. Department of Neurology - Brasil

^[2] Universidade de Sao Paulo. Faculdade de Medicina FMUSP. Department of Neurology - Brasil

^[3] Universidade de Sao Paulo. Faculdade de Medicina FMUSP. Department of Neurology - Brasil

^[4] Universidade de Sao Paulo. Faculdade de Medicina FMUSP. Department of Neurology - Brasil

^[5] Universidade de Sao Paulo. Faculdade de Medicina FMUSP. Department of Neurology - Brasil

• Document type: Journal article
• Source: MedicalExpress (São Paulo, online); v. 4, n. 5 2017-10-00.

Abstract

OBJECTIVE: Glioblastoma, the most common and lethal brain tumor, is also one of the most defying forms of malignancies in terms of treatment. Integrated genomic analysis has searched deeper into the molecular architecture of GBM, revealing a new sub-classification and promising precision in the care for patients with specific alterations. METHOD: Here, we present the classification of a Brazilian glioblastoma cohort into its main molecular subtypes. Using a high-throughput DNA sequencing procedure, we have classified this cohort into proneural, classical and mesenchymal sub-types. Next, we tested the possible use of the overexpression of the EGFR and CHI3L1 genes, detected through immunohistochemistry, for the identification of the classical and mesenchymal subtypes, respectively. RESULTS: Our results demonstrate that genetic identification of the glioblastoma subtypes is not possible using single targeted mutations alone, particularly in the case of the Mesenchymal subtype. We also show that it is not possible to single out the mesenchymal cases through CHI3L1 expression. CONCLUSION: Our data indicate that the Mesenchymal subtype, the most malignant of the glioblastomas, needs further and more thorough research to be ensure adequate identification. (AU)

• FAPESP's process: 13/02162-8 - Molecular pathogenesis and characterization of monogenic developmental diseases: a route to translational medicine
• Grantee: Berenice Bilharinho de Mendonça
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 13/06315-3 - The role of microglia activation in human astrocytoma
• Grantee: Suely Kazue Nagahashi Marie
• Support Opportunities: Regular Research Grants
• FAPESP's process: 16/15652-1 - Impact of early molecular changes in innate immune cells associated with human glioma progression
• Grantee: Thais Fernanda de Almeida Galatro
• Support Opportunities: Scholarships in Brazil - Post-Doctoral