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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Knee osteoarthritis induces atrophy and neuromuscular junction remodeling in the quadriceps and tibialis anterior muscles of rats

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Author(s):
Cunha, Jonathan Emanuel [1] ; Barbosa, Germanna Medeiros [1] ; Tome de Souza Castro, Paula Aiello [1] ; Ferreira Luiz, Beatriz Leite [1] ; Arcari Silva, Andreza Cristine [1] ; Russo, Thiago Luiz [1] ; Vasilceac, Fernando Augusto [2] ; Cunha, Thiago Mattar [3] ; Cunha, Fernando Queiroz [3] ; Salvini, Tania Fatima [1]
Total Authors: 10
Affiliation:
[1] Univ Fed Sao Carlos, Phys Therapy Dept, Sao Carlos, SP - Brazil
[2] Univ Fed Sao Carlos, Gerontol Dept, Sao Carlos, SP - Brazil
[3] Univ Sao Paulo, Pharmacol Dept, Ribeirao Preto, SP - Brazil
Total Affiliations: 3
Document type: Journal article
Source: SCIENTIFIC REPORTS; v. 9, APR 24 2019.
Web of Science Citations: 1
Abstract

Knee osteoarthritis (KOA) is associated with muscle weakness, but it is unclear which structures are involved in the muscle changes. This study assessed morphological alterations and the expression of genes and proteins linked to muscular atrophy and neuromuscular junctions (NMJs) in KOA, induced by anterior cruciate ligament transection (ACLT) in rats. Two groups of rats were assessed: control (without intervention) and KOA (ACLT surgery in the right knee). After 8 weeks, quadriceps, tibialis anterior (TA) and gastrocnemius muscles were analyzed (area of muscle fibers, NMJ, gene and protein expression). KOA group showed atrophy in quadriceps (15.7%) and TA (33%), with an increase in atrogin-1 and muscle RING-finger protein-1 (MuRF-1). KOA group showed quadriceps NMJ remodeling (reduction area and perimeter) and decrease in NMJ diameter in TA muscle. The expression of nicotinic acetylcholine receptor (nAChR) gamma-nAChR increased and that of alpha-nAChR and muscle specific tyrosine kinase (MuSK) declined in the quadriceps, with a decrease in epsilon-nAChR in TA. MuRF-1 protein expression increased in quadriceps and TA, with no changes in neural cell adhesion molecule (NCAM). In conclusion, ACLT-induced KOA promotes NMJ remodeling and atrophy in quadriceps and TA muscles, associated with inflammatory signs and changes in muscle gene and protein expression. (AU)

FAPESP's process: 16/24666-6 - Morphological and neuromuscular junction signaling pathways changes in rats with knee osteoarthritis induced by anterior cruciate ligament transection
Grantee:Jonathan Emanuel da Cunha
Support type: Scholarships in Brazil - Master
FAPESP's process: 15/26567-2 - THE EFFECT OF CRYOTHERAPY IN THE NOCICEPTION, INFLAMMATORY CYTOKINES AND NEUROMUSCULAR SIGNALING PATHWAYS OF MICE WITH KNEE ARTHRITIS
Grantee:Paula Aiello Tomé de Souza Castro
Support type: Scholarships in Brazil - Post-Doctorate