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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Effectiveness of rhBMP-2 association to autogenous, allogeneic, and heterologous bone grafts

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Author(s):
Gonzaga, Miliane Goncalves [1] ; dos Santos Kotake, Bruna Gabriela [1] ; Tocchini de Figueiredo, Fellipe Augusto [1] ; Feldman, Sara [2] ; Ervolino, Edilson [3] ; Gorita dos Santos, Maria Cecilia [4] ; Mardegan Issa, Joao Paulo [4]
Total Authors: 7
Affiliation:
[1] Univ Sao Paulo, Ribeirao Preto Med Sch, Dept Biomech Med & Rehabil Locomotor, Sao Paulo - Brazil
[2] Natl Rosario Univ, Sch Med, LABOATEM Osteoarticular Biol Tissue Engn & Emergi, Santa Fe - Argentina
[3] Sao Paulo State Univ, Fac Dent, Dept Basic Sci, Sao Paulo - Brazil
[4] Univ Sao Paulo, Sch Dent Ribeirao Preto, Dept Pediat Dent, Sao Paulo - Brazil
Total Affiliations: 4
Document type: Journal article
Source: MICROSCOPY RESEARCH AND TECHNIQUE; v. 82, n. 6, p. 689-695, JUN 2019.
Web of Science Citations: 1
Abstract

Proteins with osteoinductive potential, especially recombinant human bone morphogenetic protein (rhBMP)-2, have large effects on cell growth and their differentiation. The aim of this study was to assess repair of bone defects in rat calvaria with different types of grafts associated with rhBMP-2, through immunohistochemistry and micro computed tomography (CT) analyses. A total of 35 male Wistar rats were selected, each weighing similar to 250 g, with a waiting period of 6 weeks from the creation of the defect to the sacrifice, and divided into five groups (n = 7): autograft plus 5 mu g rhBMP-2 (AuG/BMP-2); allograft plus 5 mu g rhBMP-2 (AlG/BMP-2); xenograft (heterologous) plus 5 mu g rhBMP-2 (XeG/BMP-2); 5 mu g rhBMP-2 (BMP-2) and the control group (n = 7). The micro CT reveal that all groups associating different bone grafts with BMP-2 showed increased bone formation compared to the control. The immunostaining show that osteocalcin and bone sialoprotein were higher in groups with BMP-2 than control group; BMP was high expressed in AuG/BMP-2, AlG/BMP-2, and BMP-2; vascular endothelial growth factor (VEGF) was more expressed in groups with BMP-2; VEGF-R2 was low to moderate in AuG/BMP-2, XeG/BMP-2, and BMP-2, predominantly moderate in AlG/BMP-2 and low in the control; CD-31 was predominantly moderate in AuG/BMP-2, AlG/BMP-2, and XeG/BMP-2, low to moderate in BMP-2 and low in the control. The results revealed that rhBMP-2 improved bone repair when administered alone, or when associated with different bone grafts. (AU)

FAPESP's process: 11/01512-0 - Evaluation on the interaction between two osteogenic proteins and different bone grafts in the bone healing process of calvarial tissue in Wistar rats
Grantee:João Paulo Mardegan Issa
Support Opportunities: Regular Research Grants