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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Inactivation of Streptococcus mutans genes lytST and dltAD impairs its pathogenicity in vivo

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Castillo Pedraza, Midian C. [1] ; Rosalen, Pedro L. [2] ; Freire de Castilho, Aline Rogeria [2, 3] ; Freires, Irian de Almeida [4, 2] ; Leite, Luana de Sales [1] ; Faustoferri, Roberta C. [5] ; Quivey, Jr., Robert G. [5] ; Klein, I, Marlise
Total Authors: 8
[1] I, Sao Paulo State Univ Unesp, Dept Dent Mat & Prosthodont, Sch Dent, Rua Humaita 1680, BR-14801903 Araraquara, SP - Brazil
[2] Univ Estadual Campinas, Dept Physiol Sci, Piracicaba Dent Sch, UNICAMP, Piracicaba - Brazil
[3] Univ Estadual Campinas, Dept Pediat Dent, Piracicaba Dent Sch, UNICAMP, Piracicaba - Brazil
[4] Univ Florida, Coll Dent, Dept Oral Biol, Gainesville, FL 32610 - USA
[5] Univ Rochester, Ctr Oral Biol, Rochester, NY - USA
Total Affiliations: 5
Document type: Journal article
Source: JOURNAL OF ORAL MICROBIOLOGY; v. 11, n. 1 JAN 1 2019.
Web of Science Citations: 0

Background: Streptococcus mutans orchestrates the development of a biofilm that causes dental caries in the presence of dietary sucrose, and, in the bloodstream, S. mutans can cause systemic infections. The development of a cariogenic biofilm is dependent on the formation of an extracellular matrix rich in exopolysaccharides, which contains extracellular DNA (eDNA) and lipoteichoic acids (LTAs). While the exopolysaccharides are virulence markers, the involvement of genes linked to eDNA and LTAs metabolism in the pathogenicity of S. mutans remains unclear. Objective and Design: In this study, a parental strain S. mutans UA159 and derivative strains carrying single gene deletions were used to investigate the role of eDNA (Delta lytS and Delta lytT), LTA (Delta dltA and Delta dltD), and insoluble exopolysaccharides (Delta gtfB) in virulence in a rodent model of dental caries (rats) and a systemic infection model (Galleria mellonella larvae). Results: Fewer carious lesions were observed on smooth and sulcal surfaces of enamel and dentin of the rats infected with increment lytS, increment dltD, and Delta gtfB (vs. the parental strain). Moreover, strains carrying gene deletions prevented the killing of larvae (vs. the parental strain). Conclusions: Altogether, these findings indicate that inactivation of lytST and dltAD impaired S. mutans cariogenicity and virulence in vivo. (AU)

FAPESP's process: 14/01723-9 - Characterization of a glass ionomer cement containing natural products: in vitro and in vivo studies
Grantee:Aline Rogéria Freire de Castilho
Support Opportunities: Scholarships in Brazil - Post-Doctoral
FAPESP's process: 17/00753-0 - In vivo virulence analyses of Streptococcus mutans lytTS genes and operon dltABCD and modulation thereof by targeted therapy
Grantee:Midian Clara Castillo Pedraza
Support Opportunities: Scholarships in Brazil - Doctorate
FAPESP's process: 14/05423-0 - Role of extracellular DNA and lipoteichoic acid in the matrix of cariogenic biofilm
Grantee:Marlise Inêz Klein Furlan
Support Opportunities: Research Grants - Young Investigators Grants