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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Comparative Study of Candida albicans Inactivation by Nonthermal Plasma on Stainless Steel with and without Diamond-like Carbon Film

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Author(s):
Menegatti de Oliveira, Simone Maria [1, 2] ; da Silva, Newton Soares [3] ; Sene, Ana [1] ; Gandra, Rinaldo Ferreira [2] ; Boff Junges, Daniele Schaab [2] ; Ramirez Ramos, Marco Antonio [1] ; Vieira, Lucia [1]
Total Authors: 7
Affiliation:
[1] Univ Paraiba Valley, Lab Nanotechnol & Plasma Proc, BR-12244000 Sao Jose Dos Campos, SP - Brazil
[2] Western Parana State Univ, Lab Mycol, BR-85819110 Cascavel, PR - Brazil
[3] Univ Paraiba Valley, Lab Cell Biol & Tissue, BR-12244000 Sao Jose Dos Campos, SP - Brazil
Total Affiliations: 3
Document type: Journal article
Source: ACS OMEGA; v. 4, n. 4, p. 6891-6902, APR 2019.
Web of Science Citations: 0
Abstract

This paper reports the efficacy of nonthermal plasma (NTP) as a biocidal agent to treat 304 stainless steel (SS304) covered with a diamond-like carbon (DLC) film contaminated with Candida albicans biofilms. The association of two techniques using electric plasma was used. The first was plasma-enhanced chemical vapor deposition (PECVD) used to deposit a DLC film on the SS304. The second was NTP used on the surface of the SS304 with and without the DLC film. The combination of the SS304 surface with the DLC film was demonstrated to be effective when using the DLC film as corrosion protection. Comparing the proliferation of Candida species on the DLC film and SS304 surface, it was possible to conclude that Candida species stays longer in the DLC film than in SS304. The reduction of colony numbers was visible after 5 min using plasma on both surfaces; in addition, 99% of Candida species were eliminated after 15 min. Three C. albicans microorganisms were used. Two were from samples of urine and tracheal secretion, and one was from the American Type Culture Collection (ATCC \#90028). Characterization of the plasma chemical species was performed using optical emission spectroscopy in order to understand the nature of the chemical species that inactivated the microorganisms. The DLC film was analyzed using profilometry, Raman spectroscopy, scanning electron microscopy, and tribocorrosion tests. The tribocorrosion tests were used to evaluate the effectiveness of the DLC film in protecting the SS304 surface against corrosion in simulated body fluid because corrosion species from the SS304 could interfere in biofilm growth and mask the effect of the plasma. The results of the factorial analysis of variance confirmed the statistical significance (p > 0.05) of the plasma as a biocidal agent, considering the reduction of colony-forming units of C. albicans. It was found that exposure of the samples to the plasma for only 5 min resulted in reductions ranging from 96.4 to 100.0% for all the microorganisms studied. (AU)

FAPESP's process: 13/20054-8 - Studies of adhesion, proliferation and cell death in the interaction of cells with nanostructured materials
Grantee:Newton Soares da Silva
Support Opportunities: Regular Research Grants
FAPESP's process: 17/10491-2 - Production of multifunctional coatings for high performance as solid lubricant and corrosion protection for aerospace components and biomedical devices
Grantee:Lúcia Vieira
Support Opportunities: Regular Research Grants