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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Aedes aegypti saliva impairs M1-associated proinflammatory phenotype without promoting or affecting M2 polarization of murine macrophages

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Author(s):
Barros, Michele S. [1] ; Lara, Priscila G. [1] ; Fonseca, Monique T. [2] ; Moretti, Eduardo H. [2] ; Filgueiras, Luciano R. [3] ; Martins, Joilson O. [4] ; Capurro, Margareth L. [5, 6] ; Steiner, Alexandre A. [2] ; Sa-Nunes, Anderson [6, 1]
Total Authors: 9
Affiliation:
[1] Univ Sao Paulo, Inst Biomed Sci, Dept Immunol, Lab Expt Immunol, BR-05508000 Sao Paulo, SP - Brazil
[2] Univ Sao Paulo, Inst Biomed Sci, Dept Immunol, Lab Sepsis Neurobiol, BR-05508000 Sao Paulo, SP - Brazil
[3] Univ Sao Paulo, Inst Biomed Sci, Dept Immunol, Lab Immunopharmacol, BR-05508000 Sao Paulo, SP - Brazil
[4] Univ Sao Paulo, Sch Pharmaceut Sci, Dept Clin & Toxicol Anal, Lab Immunoendocrinol, BR-05508000 Sao Paulo, SP - Brazil
[5] Univ Sao Paulo, Inst Biomed Sci, Dept Parasitol, Lab Genetically Modified Mosquitoes, BR-05508000 Sao Paulo, SP - Brazil
[6] Natl Council Sci & Technol Dev, Natl Inst Sci & Technol Mol Entomol, INCT EM CNPq, Rio De Janeiro, RJ - Brazil
Total Affiliations: 6
Document type: Journal article
Source: PARASITES & VECTORS; v. 12, MAY 16 2019.
Web of Science Citations: 1
Abstract

BackgroundDuring the feeding process, the mouthparts of hematophagous mosquitoes break the skin barrier and probe the host tissue to find the blood. The saliva inoculated in this microenvironment modulates host hemostasis, inflammation and adaptive immune responses. However, the mechanisms involved in these biological activities remain poorly understood and few studies explored the potential roles of mosquito saliva on the individual cellular components of the immune system. Here, we report the immunomodulatory activities of Aedes aegypti salivary cocktail on murine peritoneal macrophages.ResultsThe salivary gland extract (SGE) of Ae. aegypti inhibited the production of nitric oxide and inflammatory cytokines such as interleukin-6 (IL-6) and IL-12, as well as the expression of inducible nitric oxide synthase and NF-B by murine macrophages stimulated by lipopolysaccharide (LPS) plus interferon- (IFN-). The spare respiratory capacity, the phagocytic and microbicidal activities of these macrophages were also reduced by Ae. aegypti SGE. These phenotypic changes are consistent with SGE suppressing the proinflammatory program of M1 macrophages. On the other hand, Ae. aegypti SGE did not influence M2-associated markers (urea production, arginase-1 and mannose receptor-1 expression), either in macrophages alternatively activated by IL-4 or in those classically activated by LPS plus IFN-. In addition, Ae. aegypti SGE did not display any cytokine-binding activity, nor did it affect macrophage viability, thus excluding supposed experimental artifacts.ConclusionsGiven the importance of macrophages in a number of biological processes, our findings help to enlighten how vector saliva modulates vertebrate host immunity. (AU)

FAPESP's process: 16/04921-1 - Arterial tonus in septic shock: a new facet to an old problem.
Grantee:Alexandre Alarcon Steiner
Support type: Regular Research Grants
FAPESP's process: 17/11540-7 - Investigating the role of insulin in the presence of allergic pulmonary inflammation in diabetic and healthy mice
Grantee:Joilson de Oliveira Martins
Support type: Regular Research Grants
FAPESP's process: 13/12467-0 - Effects of Aedes aegypti salivary components on the biology of macrophages and potential therapeutical applications.
Grantee:Michele Silva de Barros
Support type: Scholarships in Brazil - Doctorate
FAPESP's process: 15/22934-0 - Immunomodulation of experimental autoimmune encephalomyelitis by salivary proteins from Aedes aegypti mosquito.
Grantee:Anderson de Sá Nunes
Support type: Regular Research Grants