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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Purification and characterization of FtsZ from the citrus canker pathogen Xanthomonas citri subsp. citri

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Kopacz, Malgorzata M. [1, 2] ; Lorenzoni, Andre S. G. [1] ; Polaquini, Carlos R. [3] ; Regasini, Luis O. [3] ; Scheffers, Dirk-Jan [1]
Total Authors: 5
[1] Univ Groningen, Groningen Biomol Sci & Biotechnol Inst, Dept Mol Microbiol, Groningen - Netherlands
[2] Univ Southern Denmark, Dept Chem Engn Biotechnol & Environm Technol, Odense M - Denmark
[3] Sao Paulo State Univ UNESP, Inst Biosci Humanities & Exact Sci, Dept Chem & Environm Sci, Lab Antibiot & Chemotherapeut, Sao Jose Do Rio Preto, SP - Brazil
Total Affiliations: 3
Document type: Journal article
Source: MICROBIOLOGYOPEN; v. 8, n. 5 MAY 2019.
Web of Science Citations: 1

Xanthomonas citri subsp. citri (Xac) is the causative agent of citrus canker, a plant disease that significantly impacts citriculture. In earlier work, we showed that alkylated derivatives of gallic acid have antibacterial action against Xac and target both the cell division protein FtsZ and membrane integrity in Bacillus subtilis. Here, we have purified native XacFtsZ and characterized its GTP hydrolysis and polymerization properties. In a surprising manner, inhibition of XacFtsZ activity by alkyl gallates is not as strong as observed earlier with B. subtilis FtsZ. As the alkyl gallates efficiently permeabilize Xac membranes, we propose that this is the primary mode of antibacterial action of these compounds. (AU)

FAPESP's process: 14/18330-0 - Synthesis and biological evaluation of curcumin-cinnamaldehyde hybrids as bacterial cell division inhibitors
Grantee:Luis Octávio Regasini
Support type: Regular Research Grants
FAPESP's process: 13/50367-8 - New environmental-friendly compounds to combat citrus canker
Grantee:Henrique Ferreira
Support type: Regular Research Grants